Arch Virol (2006) 151: 2077–2083
Complete nucleotide sequences of three dsRNA segments
from Raphanus sativus-root cv. Yipinghong with leaf
yellow edge symptoms
, J. S. Chen
, H. Zhang
, and S. N. Chen
Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou, China
College of Life Sciences, Zhejiang University, Hangzhou, China
College of Bio-Safety Science and Technology, Hunan Agricultural University,
Received January 13, 2006; accepted March 9, 2006
Published online April 28, 2006
Summary. The two minor dsRNA bands, previously detected in symptomatic
leaves of Raphanus sativus-root cv.Yipinghong , were subjected to further anal-
ysis. cDNA cloning and sequencing revealed that the smaller of the two dsRNA
bands is actually a doublet consisting of two co-migrating dsRNA segments and
the resulting three segments were designated as RasR 3, RasR 4, and RasR 5.
RasR 3 was 1717 bp in length and potentially encoded a protein of about 55.3 kDa,
containing all of the six conserved motifs shared by the RNA dependent RNA
polymerases of members of the family Partitiviridae. RasR 4 and RasR 5, which
co-migrated in the 5% polyacrylamide gel, were 1521 and 1485 bp in length and
each encoded a putative protein of unknown function. Their molecular masses, as
calculated from the deduced amino acid, were 38.2 and 38.8 kDa, respectively.
UTRs of all three segments shared regions of high sequence similarities,
but were distinct from those of the RasR 1 and RasR 2. Taken together, these results
along with those described in the previous report , suggest that more than one
partitivirus was co-infecting radish leaves.
In the previous report , we illustrated the presence of four distinct dsRNA
bands in radish leaves collected from Hangzhou, China. Extraction of virus-like
The sequences reported in this paper have been submitted to the GenBank database and
have been assigned the accession numbers DQ218036, DQ218037, and DQ218038.