Arch Virol (2004) 149: 773–777
Complete nucleotide sequences of genome
segments 1 and 3 of Rosellinia anti-rot virus
in the family Reoviridae
C. Z. Wei
, H. Osaki
, T. Iwanami
, N. Matsumoto
, and Y. Ohtsu
National Institute of Fruit Tree Science, Tsukuba, Japan
National Institute for Agro-Environmental Sciences, Tsukuba, Japan
Received September 18, 2003; accepted October 20, 2003
Published online December 8, 2003
Summary. The nucleotide sequences of genome segments 1 and 3 of Rosellinia
anti-rot virus (RArV) from a hypovirulent isolate, W370, of the plant pathogen
Rosellinia necatrix were determined. The complete nucleotide sequence of the
genome segment 1 encoded a putative RNA-dependent RNA polymerase (RDRP).
The deduced amino acid sequence of RDRP of RArV showed 29% identity with
RDRPs of Colorado tick fever virus (CTFV) and European Eyach virus (EYAV)
in the genus Coltivirus, and identities of 23–21% with members of the genera
Fijivirus and Cypovirus. Both RArV and the Coltivirus member might have
originated from a common virus ancestor.
Rosellinia anti-rot virus (RArV), a new member of the family Reoviridae,is
isolated for the ﬁrst time from a hypovirulent isolate, W370, of the plant pathogen
Rosellinia necatrix Prillieux . Morphological characterization of RArV and
sequence analysis of most genome segments had suggested that RArV was
presumably constitutive of a new genus of the family Reoviridae [11, 15].
However, the incomplete nature of the RNA sequence data of RArV did
not clarify fully the genetic relationship among other members of the family
Reoviridae. In this report, the complete nucleotide sequences of the genome
The nucleotide sequence data reported in this paper for segment 1 and segment 3 of RArV
will appear in the GenBank/EMBL/DDBJ nucleotide sequence databases under accession
numbers AB102674 and AB102675, respectively.