Arch Virol (2007) 152: 2131–2134 DOI 10.1007/s00705-007-1043-9 Printed in The Netherlands Annotated Sequence Record Complete nucleotide sequences of cotton leaf curl Rajasthan virus and its associated DNA b molecule infecting tomato M. S. Shahid, S. Mansoor, R. W. Briddon Plant Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan Received 5 April 2007; Accepted 8 July 2007; Published online 16 August 2007 # Springer-Verlag 2007 Tomato leaf curl disease (ToLCD) is a serious prob- exhibited an unusually severe ‘‘leaf curl’’ pheno- lem throughout the warmer parts of the world . type consisting of highly curled leaves, reduced in On the Indian subcontinent, the disease is caused size with frond-like enations on the veins on their by a diverse range of single-stranded DNA viruses undersides. Total nucleic acids were extracted as of the genus Begomovirus (family Geminiviridae) described previously . Initial analysis by PCR . The virus ﬁrst identiﬁed causing the disease with universal primers  and limited sequencing on the sub-continent in the 1990 s, tomato leaf curl showed two of the plants to be infected with New Delhi virus (ToLCNDV), is a typical bipartite ToLCNDV (results not shown). Two primer pairs, begomovirus; having two genomic components,
Archives of Virology – Springer Journals
Published: Oct 1, 2007
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