Arch Virol (2002) 147: 865–870
Complete nucleotide sequence of the genomic RNA
of Aconitum latent virus (genus Carlavirus) isolated
from Delphinium sp.
, H. Yamamoto
, M. Inoue
, K. Yamashita
, H. Furuya
, and H. Naito
Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Japan
Akita Prefectural Center of Biological Resources Development, Akita, Japan
Aomori Green Bio Center, Aomori, Japan
Accepted May 15, 2001
Summary. We determined the complete nucleotide sequence of Aconitum latent
virus (AcLV-D) isolated from Delphinium sp. The genomic RNA of AcLV-D
is 8,657 nucleotides in length, excluding the poly (A) tail. Based on computer
analysis, six open reading frames (ORFs) were identiﬁed as for other carlaviruses.
Although each ORF differs from those of previously reported carlaviruses, the
coat protein of AcLV and Potato virus M (PVM) shows a high level of identity.
The results suggest that AcLV is a distinct carlavirus species but closely related
Delphinium sp. is grown in Japan as an ornamental plant for cut ﬂowers.Although
the crop is propagated vegetatively in Japan, tubers of new varieties are con-
tinuously being imported from Europe. Recently, virus-like diseases have been
occurred in Delphinium sp. grown in greenhouses. The disease, in which faint
mosaic on leaves occurs and sometimes spots on petals, can lead to signiﬁcant
quality deterioration. The leaf symptoms are very similar to those when Aconitum
napellus, which belong to the same family (Ranunculaceae) as Delphinium,isin-
fected with Aconitum latent carlavirus (AcLV) . Therefore, we attempted to
Sequence data from this article has been deposited with the DDBJ/EMBL/GenBank
database under accession number AB051848.