Arch Virol (2003) 148: 165–175
Complete nucleotide sequence of spring beauty latent virus,
a bromovirus infectious to Arabidopsis thaliana
K. Fujisaki, F. Hagihara, M. Kaido, K. Mise, and T. Okuno
Laboratory of Plant Pathology, Graduate School of Agriculture,
Kyoto University, Kyoto, Japan
Received May 20, 2002; accepted August 20, 2002
Published online November 4, 2002
Summary. Spring beauty latent virus (SBLV), a bromovirus, systemically and
efﬁciently infected Arabidopsis thaliana, whereas the well-studied bromoviruses
brome mosaic virus (BMV) and cowpea chlorotic mottle virus (CCMV) did not
infect and poorly infected A. thaliana, respectively. We constructed biologically
active cDNA clones of SBLV genomic RNAs and determined their complete
nucleotide sequences. Interestingly, SBLV RNA3 contains both the box B motif
in the intercistronic region, as does BMV, and the subgenomic promoter-like
sequence in the 5
noncoding region, as does CCMV. Sequence comparisons of
SBLV, BMV, CCMV, and broad bean mottle virus demonstrated that SBLV is
closely related to BMV and CCMV.
Systemic infection by plant viruses is established via multiple steps.At each step,
several viral and host factors are involved in virus multiplication. To elucidate the
mechanism of viral infections of plants, many viral factors have been examined,
but host factors have not been identiﬁed in sufﬁcient detail. The complete genome
of Arabidopsis thaliana has been sequenced [8, 9], and this plant species has
been used as a model in many studies including the analysis of plant–microbe
interactions [14, 15].
Bromovirusesareicosahedralplant viruses, the genomes ofwhich are positive-
sense tripartite RNA molecules designated RNA1, RNA2, and RNA3 .
Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases
under the accession numbers AB080598, AB080599 and AB080600.