Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China

Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from Taian, China (LChV-1-TA), detected by small-RNA deep sequencing and amplified by overlapping RT-PCR. The LChV-1-TA genome was 16,932 nt in length and contained nine open reading frames (ORFs), with sequence identity at the overall genome level of 76 %, 76 %, and 78 % to LChV-1 isolates Y10237 (UW2 isolate), EU715989 (ITMAR isolate) and JX669615 (V2356 isolate), respectively. Based on the phylogenetic analysis of HSP70h amino acid sequences of Closteroviridae family members, LChV-1-TA was grouped into a well-supported cluster with the members of the genus Velarivirus and was also closely related to other LChV-1 isolates. This is the first report of the complete nucleotide sequence of LChV-1 infecting sweet cherry in China. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China

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Publisher
Springer Journals
Copyright
Copyright © 2016 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2737-z
Publisher site
See Article on Publisher Site

Abstract

Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from Taian, China (LChV-1-TA), detected by small-RNA deep sequencing and amplified by overlapping RT-PCR. The LChV-1-TA genome was 16,932 nt in length and contained nine open reading frames (ORFs), with sequence identity at the overall genome level of 76 %, 76 %, and 78 % to LChV-1 isolates Y10237 (UW2 isolate), EU715989 (ITMAR isolate) and JX669615 (V2356 isolate), respectively. Based on the phylogenetic analysis of HSP70h amino acid sequences of Closteroviridae family members, LChV-1-TA was grouped into a well-supported cluster with the members of the genus Velarivirus and was also closely related to other LChV-1 isolates. This is the first report of the complete nucleotide sequence of LChV-1 infecting sweet cherry in China.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2016

References

  • Molecular characterization and taxonomy of grapevine leafroll-associated virus 7
    Jelkmann, W; Mikona, C; Turturo, C; Navarro, B; Rott, ME; Menzel, W; Saldarelli, P; Minafra, A; Martelli, GP
  • A multiplex RT-PCR assay for simultaneous detection of four viruses from sweet cherry
    Zong, X; Wang, W; Wei, H; Wang, J; Chen, X; Xu, L; Zhu, D; Tan, Y; Liu, Q

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