Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China

Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from Taian, China (LChV-1-TA), detected by small-RNA deep sequencing and amplified by overlapping RT-PCR. The LChV-1-TA genome was 16,932 nt in length and contained nine open reading frames (ORFs), with sequence identity at the overall genome level of 76 %, 76 %, and 78 % to LChV-1 isolates Y10237 (UW2 isolate), EU715989 (ITMAR isolate) and JX669615 (V2356 isolate), respectively. Based on the phylogenetic analysis of HSP70h amino acid sequences of Closteroviridae family members, LChV-1-TA was grouped into a well-supported cluster with the members of the genus Velarivirus and was also closely related to other LChV-1 isolates. This is the first report of the complete nucleotide sequence of LChV-1 infecting sweet cherry in China. Archives of Virology Springer Journals

Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China

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Springer Vienna
Copyright © 2016 by Springer-Verlag Wien
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
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