Arch Virol (2009) 154:365–368 DOI 10.1007/s00705-008-0296-2 ANNOTATED SEQUENCE RECORD Complete nucleotide sequence of chili leaf curl virus and its associated satellites naturally infecting potato in Pakistan M. Mubin Æ R. W. Briddon Æ S. Mansoor Received: 3 July 2008 / Accepted: 4 December 2008 / Published online: 4 January 2009 Springer-Verlag 2009 A severe leaf curl disease was observed in 2005 on potato Five symptomatic potato plants were randomly selected (Solanum tuberosum L.) crops in Lahore, Punjab province, for further analysis. Total DNA was extracted using CTAB Pakistan. Affected plants were severely stunted with apical , and universal primers were used to PCR amplify full- leaf curl and crinkled leaves, symptoms typical of virus length begomovirus, betasatellite and alphasatellite mole- infection. Potato is an important food crop grown both in the cules [3, 6, 13]. Ampliﬁed PCR products of expected sizes plains and hilly areas of Pakistan. Viral diseases are important were cloned into pTZ57R/T (Fermentas) vector and com- constraints on this crop and are usually managed by mani- pletely sequenced. Sequences were assembled and analyzed pulation of planting dates and use of virus-free seed potato. with the Lasergene sequence analysis software (DNA Star The crop is affected by
Archives of Virology – Springer Journals
Published: Feb 1, 2009
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