Arch Virol (2008) 153:755–757 DOI 10.1007/s00705-008-0040-y ANNOTATED SEQUENCE RECORD Complete nucleotide sequence of a South African isolate of grapevine leafroll-associated virus 3 reveals a 5 UTR of 737 nucleotides H. J. Maree Æ M.-J. Freeborough Æ J. T. Burger Received: 9 November 2007 / Accepted: 27 December 2007 / Published online: 15 February 2008 Springer-Verlag 2008 Grapevine leafroll-associated virus 3 (GLRaV-3) is the the NY-1 isolate. However, we were unable to reach the type species of the genus Ampelovirus (family Clostero- reported 5 -end of the NY-1 isolate and consistently found viridae). It is an economically important virus that is amplicons that started at +50 nt. After further experimen- known to only infect Vitis spp. and that has a negative tation with PCR conditions, a range of amplicons were impact on the wine and table grape industries worldwide. generated that extended beyond the NY-1 sequence’s To date, there has been only one report that claims the 5 -end. A possible explanation for this inconsistency, using complete nucleotide sequence of GLRaV-3, by Ling et al. poly(A) tailing, might be the high uracil content in the 0 0 in 2004 (isolate NY-1, AF037268) . In that report the 5 5 -region
Archives of Virology – Springer Journals
Published: Apr 1, 2008
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