Complete nucleotide sequence of a California isolate of Radish mosaic virus

Complete nucleotide sequence of a California isolate of Radish mosaic virus Arch Virol (2008) 153:2167–2168 DOI 10.1007/s00705-008-0229-0 ANNOTATED SEQUENCE RECORD Complete nucleotide sequence of a California isolate of Radish mosaic virus Ken Komatsu Æ Kouji Hatada Æ Masayoshi Hashimoto Æ Johji Ozeki Æ Kensaku Maejima Æ Satoshi Kagiwada Æ Yasuyuki Yamaji Æ Shigetou Namba Received: 10 September 2008 / Accepted: 6 October 2008 / Published online: 31 October 2008 Springer-Verlag 2008 Radish mosaic virus (RaMV) is a member of the genus California isolate of RaMV (RaMV-CA), as well as its Comovirus of the family Comoviridae [9]. Comoviruses comparison with RaMV-J. have two positive-, single-stranded genomic RNAs (RNA1 RaMV-CA (ATCC PV-138) was originally isolated and RNA2), which are encapsidated into separate spherical from infected leaves of turnip (Brassica rapa) in Califor- virions. RaMV mainly infects cruciferous plants, unlike nia, USA [1]. It was propagated in radish (Raphanus most members of the genus Comovirus, which predomi- sativus) and purified as described [8]. Viral RNA extracted nantly infect plants of the Leguminosae family. RaMV was from the purified virions was used as a template for cDNA originally identified in California, USA, in 1939 [12], but synthesis with an oligo (dT) primer and for 5 -RACE. its occurrence has also been reported in Europe, Archives of Virology Springer Journals

Complete nucleotide sequence of a California isolate of Radish mosaic virus

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Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
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