Arch Virol (2001) 146: 2443–2453
Complete nucleotide sequence and genome organization
of Beet soilborne mosaic virus,
a proposed member of the genus Benyvirus
, E. B. Telford
, J. S. Batten
, K.-B. G. Scholthof
, and C. M. Rush
Department of Plant Pathology and Microbiology, Texas A&M Agricultural
Research Center, Amarillo, Texas, U.S.A.
Department of Plant Pathology and Microbiology, Texas A&M University,
College Station, Texas, U.S.A.
Accepted August 2, 2001
Summary. The complete nucleotide sequences of RNAs 1 to 4 of Beet soilborne
mosaic virus (BSBMV) were determined. The genomic organization of BSBMV
is identical to Beet necrotic yellow vein virus (BNYVV), the type species of
the genus Benyvirus. BSBMV RNA1 encodes a single large open reading frame
(ORF) with similar replicase-associated motifs identiﬁed for BNYVV. BSBMV
RNA2 has six potential ORFs with an organization resembling BNYVV RNA2.
RNA3 and RNA4 resemble the analogous BNYVV RNAs, which encode proteins
associated with symptom development and fungal transmission, respectively. The
predicted ORFs on BNYVV and BSBMV reveal 23% to 83% amino acid identity
and the overall nucleotide sequences are 35% to 77% identical. Based on sequence
analyses, BSBMV is a new benyvirus that can be distinguished from BNYVV.
Beet necrotic yellow vein virus (BNYVV), the type species of the Benyvirus
genus, is the causal agent of rhizomania in sugar beet (Beta vulgaris L.) .
Rhizomania is characterized by massive lateral root proliferation, constriction of
The nucleotide sequence data described in this paper were deposited as GenBank acces-
sion numbers AF280539 (RNA1), AF061869 (RNA2), AF280540 (RNA3) and AF280541
Present address: USDA/ARS and Department of Plant Pathology, Cornell University,
Ithaca, NY 14853, U.S.A.