Arch Virol (2004) 149: 625–632
Complete genomic sequence of Pepper vein banding
virus (PVBV): a distinct member of the genus Potyvirus
R. Anindya, J. Joseph, T.D.S. Gowri, and H.S. Savithri
Department of Biochemistry, Indian Institute of Science, Bangalore, India
Received July 9, 2003; accepted September 23, 2003
Published online November 13, 2003
Summary. The complete genomic sequence of Pepper vein banding virus
(PVBV), a potyvirus infecting chilli and other solanaceous plants in south India,
was determined and compared with those of other potyviruses. The viral genome
contained 9711 nucleotides, excluding the poly-A tail. The length of the 5
-untranslated regions (UTR) were 163 and 281 nucleotides respectively. As for
other potyviruses, the PVBV genome has a single open reading frame (ORF)
starting at nucleotide 164 and ending at nt 9430, which encodes a polyprotein of
3088 amino acid residues. There are nine putative conserved cleavage sites within
the polyprotein, which can result in ten functionally distinct protein products.
Phylogenetic analysis of the potyviral polyprotein sequences showed that PVBV
is a distinct species of this genus.
Pepper vein banding virus (PVBV) causes major damage to the production of
pepper (Capsicum americananum) in south India . Symptoms produced by
PVBV infection on C. annuum cv. California Wonder include vein clearing, green
banding of major veins, leaf distortion and reduced leaf size. In an earlier study, 3
terminal 3.8 kb nucleotide sequence of PVBV was determined  and comparative
analysis of coat protein (CP) and 3
-untranslated region (3
UTR) suggested that
PVBV was a distinct member of the genus Potyvirus, family Potyviridae . Chilli
vein banding mottle virus (CVBMV), reported from Thailand , had signiﬁcant
similarity with PVBV and was suggested to be a strain of the same virus. In
the present study we report complete nucleotide sequence of PVBV genome and
analysis of taxonomic position based on the polyprotein sequence.
The GeneBank accession number for the sequence reported in this paper is AJ237843.