Arch Virol (2009) 154:1173–1175 DOI 10.1007/s00705-009-0405-x ANNOTATED SEQUENCE RECORD Complete genome sequences of two isolates of Kalanchoe ¨ latent virus M. Dinesen Æ M. Lundmark Æ M. Albrechtsen Received: 11 March 2009 / Accepted: 18 May 2009 / Published online: 17 June 2009 Springer-Verlag 2009 Kalanchoe¨ latent virus (KLV), which was ﬁrst reported in mut- or KV-2-infected Kalanchoe¨ blossfeldiana cv. Nadia 1982 by S. S. Hearon, belongs to the genus Carlavirus in using a slightly altered protocol  for the RNeasy Plant the family Flexiviridae . Carlaviruses have monopartite, Mini Kit (QIAGEN). The RNA was reverse transcribed, positive-sense, single-stranded, and poly(A) tailed RNA and the entire genomes of KV-1-mut and KV-2 were PCR- genomes . Carlavirus genomes range in size from ampliﬁed in 5 and 6 overlapping fragments, respectively, 8,363 nts for shallot latent virus (SLV, NC_003557) to using virus-speciﬁc primers designed on the basis of the 8,855 nts for chrysanthemum virus B (CVB, NC_009087) sequences determined from the C. quinoa-derived PCR 0 0 0 0 and comprise a total of six ORFs . Only partial sequence products. The 5 and 3 ends were ampliﬁed using a 5 /3 information on the KLV genome exists [10, 11]. Here, we
Archives of Virology – Springer Journals
Published: Jul 1, 2009
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