Complete genome sequence of marine bacterium Pseudoalteromonas phenolica bacteriophage TW1

Complete genome sequence of marine bacterium Pseudoalteromonas phenolica bacteriophage TW1 For molecular study of marine bacteria Pseudoalteromonas phenolica using bacteriophage, a novel bacteriophage, TW1, belonging to the family Siphoviridae , was isolated, and its genome was completely sequenced and analyzed. The phage TW1 genome consists of 39,940-bp-length double-stranded DNA with a GC content of 40.19 %, and it was predicted to have 62 open reading frames (ORFs), which were classified into functional groups, including phage structure, packaging, DNA metabolism, regulation, and additional function. The phage life style prediction using PHACTS showed that it may be a temperate phage. However, genes related to lysogeny and host lysis were not detected in the phage TW1 genome, indicating that annotation information about P. phenolica phages in the genome databases may not be sufficient for the functional prediction of their encoded proteins. This is the first report of a P. phenolica -infecting phage, and this phage genome study will provide useful information for further molecular research on P. phenolica and its phage, as well as their interactions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Complete genome sequence of marine bacterium Pseudoalteromonas phenolica bacteriophage TW1

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1776-6
Publisher site
See Article on Publisher Site

Abstract

For molecular study of marine bacteria Pseudoalteromonas phenolica using bacteriophage, a novel bacteriophage, TW1, belonging to the family Siphoviridae , was isolated, and its genome was completely sequenced and analyzed. The phage TW1 genome consists of 39,940-bp-length double-stranded DNA with a GC content of 40.19 %, and it was predicted to have 62 open reading frames (ORFs), which were classified into functional groups, including phage structure, packaging, DNA metabolism, regulation, and additional function. The phage life style prediction using PHACTS showed that it may be a temperate phage. However, genes related to lysogeny and host lysis were not detected in the phage TW1 genome, indicating that annotation information about P. phenolica phages in the genome databases may not be sufficient for the functional prediction of their encoded proteins. This is the first report of a P. phenolica -infecting phage, and this phage genome study will provide useful information for further molecular research on P. phenolica and its phage, as well as their interactions.

Journal

Archives of VirologySpringer Journals

Published: Jan 1, 2014

References

  • Artemis and ACT: viewing, annotating and comparing sequences stored in a relational database
    Carver, T; Berriman, M; Tivey, A; Patel, C; Bohme, U; Barrell, BG; Parkhill, J; Rajandream, MA
  • Identifying bacterial genes and endosymbiont DNA with Glimmer
    Delcher, AL; Bratke, KA; Powers, EC; Salzberg, SL
  • MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences
    Kumar, S; Nei, M; Dudley, J; Tamura, K
  • PHACTS, a computational approach to classifying the lifestyle of phages
    McNair, K; Bailey, BA; Edwards, RA

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