Arch Virol (2009) 154:1169–1172 DOI 10.1007/s00705-009-0414-9 ANNOTATED SEQUENCE RECORD Complete genome sequence of a Tobacco necrosis virus D isolate from olive trees Joana M. S. Cardoso Æ Maria R. Fe ´lix Æ Maria I. E. Clara Æ Solange Oliveira Received: 2 April 2009 / Accepted: 27 May 2009 / Published online: 9 June 2009 Springer-Verlag 2009 The ﬁrst complete genome sequence of an isolate of detected in an olive tree (Olea europaea L.) when several Tobacco necrosis virus D (TNV-D; genus Necrovirus, mechanically transmitted isolates tested positive in ELISA family Tombusviridae) was that of a bean isolate from against a wide-spectrum antiserum to TNV . One of England . The complete nucleotide sequence of another those was studied further, and its CP gene sequence and the TNV-D isolate, an Hungarian isolate from tobacco plants corresponding deduced amino acid sequence revealed a (TNV-D ), revealed a similar genomic organization . high degree of identity (86.2%) with that of TNV-D, sug- The single-stranded positive-sense RNA genome of TNV-D gesting the presence of a TNV-D isolate . However, is about 3.8 kb in size and contains six open reading frames further genomic characterization of this isolate revealed (ORFs). The two 5 -proximal
Archives of Virology – Springer Journals
Published: Jul 1, 2009
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