Complete genome sequence of a novel porcine parainfluenza virus 5 isolate in Korea

Complete genome sequence of a novel porcine parainfluenza virus 5 isolate in Korea A novel cytopathogenic paramyxovirus was isolated from a lung sample from a piglet, using continuous porcine alveolar macrophage cells. Morphologic and genetic studies indicated that this porcine virus (pPIV5) belongs to the species Parainfluenza 5 in the family Paramyxoviridae . We attempted to determine the complete nucleotide sequence of the first Korean pPIV5 isolate, designated KNU-11. The full-length genome of KNU-11 was found to be 15,246 nucleotides in length and consist of seven nonoverlapping genes (3′-N-V/P-M-F-SH-HN-L-5′) predicted to encode eight proteins. The overall degree of nucleotide sequence identity was 98.7 % between KNU-11 and PIV5 (formerly simian virus 5, SV5), a prototype paramyxovirus, and the putative proteins had 74.4 to 99.2 % amino acid identity to those of PIV5. Phylogenetic analysis further demonstrated that the novel pPIV5 isolate is a member of the genus Rubulavirus of the subfamily Paramyxovirinae . The present study describes the identification and genomic characterization of a pPIV5 isolate in South Korea. Archives of Virology Springer Journals

Complete genome sequence of a novel porcine parainfluenza virus 5 isolate in Korea

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Springer Vienna
Copyright © 2013 by Springer-Verlag Wien
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
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