ANNOTATED SEQUENCE RECORD
Complete genome sequence of a novel calicivirus from a goose
Received: 23 February 2014 / Accepted: 6 April 2014 / Published online: 23 April 2014
Ó Springer-Verlag Wien 2014
Abstract A novel goose calicivirus (GoCV) was
sequenced. The 8013-nt-long genome was organized into
two open reading frames that were in the same frame and
separated by 3 nucleotides. This feature is similar to what
has been observed in turkey calicivirus (TuCV). Compar-
ison of GoCV with other caliciviruses showed that it shared
the highest amino acid sequence identities of 62, 38, and
52 % in the nonstructural protein, VP1, and VP2, respec-
tively, with TuCV. Phylogenetic analysis based on the
amino acid sequences of nonstructural protein and VP1
demonstrated that GoCV was most closely related to but
distinct from TuCV. Thus, GoCV was identiﬁed as a novel
member in the proposed genus Nacovirus.
To date, the family Caliciviridae consists of ﬁve ofﬁcial
genera (Lagovirus, Nebovirus, Norovirus, Sapovirus, and
Vesivirus) and four proposed genera (Bavovirus, Nacovi-
rus, Recovirus, and Valovirus)[2, 3, 7, 15, 16, http://www.
caliciviridae.com/]. In addition, a novel calicivirus, ‘‘se-
calivirus’’, has recently been partially sequenced .
Caliciviruses possess single-stranded, positive-stranded,
polyadenylated RNA genomes that are organized into
either two or three major open reading frames (ORFs) .
ORF1, located close to the 5
terminus of the genome,
encodes nonstructural (NS) proteins that are present in all
caliciviruses in the same order: N-terminal protein (Nterm),
NTPase, 3A-like protein, VPg, protease (Pro), and RNA
polymerase (Pol) . In noro-, reco-, and vesiviruses,
additional ORFs, ORF2 and ORF3, encode the major
capsid protein VP1 and the minor structural protein VP2,
respectively [2, 3]. In the case of bavo-, lago-, naco-, nebo-,
sapo-, and valoviruses, the VP1 is encoded within the
ORF1, and the ORF encoding VP2 is ORF2 [2, 7, 15, 16].
Caliciviruses are known to infect a variety of hosts.
They have been detected in various birds, including
chickens , goldﬁnches , guinea fowl , pheasants
, turkeys , and white terns . To date, no cali-
civiruses have been reported in geese. In this study, we
report the complete sequence of a novel calicivirus detec-
ted from a goose.
Provenance of the virus material
A fecal sample (designated N) was collected from a
60-day-old healthy Landes goose in October 2012 in
China. The sample was treated using the method described
by Sauvage et al. . RNA was extracted using an
RNeasy Mini Kit (QIAGEN), and randomly ampliﬁed as
described previously . Random PCR products were
separated on a 1 % agarose gel, yielding a DNA smear.
The GenBank accession number for the study sequence is KJ473715.
Electronic supplementary material The online version of this
article (doi:10.1007/s00705-014-2083-6) contains supplementary
material, which is available to authorized users.
Q. Liao Á X. Wang Á D. Wang Á D. Zhang (&)
Key Laboratory of Animal Epidemiology and Zoonosis of
Ministry of Agriculture, College of Veterinary Medicine, China
Agricultural University, No. 2 Yuanmingyuan West Road,
Haidian district, Beijing 100193, People’s Republic of China
Department of Animal Science and technology, Chongqing
Three Gorges Vocational College, Chongqing,
People’s Republic of China
Arch Virol (2014) 159:2529–2531