Comparisons of the Genomes of New Giant Phages Isolated from Environmental Pseudomonas aeruginosa Strains of Different Regions

Comparisons of the Genomes of New Giant Phages Isolated from Environmental Pseudomonas aeruginosa... To study the genome diversity of bacteriophages from geographically distant natural populations, new giant ϕKZ-like Pseudomonas aeruginosa phages isolated in two different regions were compared with earlier known phages of three species (ϕKZ, Lin68, EL). A broad spectrum of lytic activity was demonstrated for all ϕKZ-like phages. Phages of the ϕKZ species proved to be common in natural populations of various regions, while EL- and Lin68-related phages were extremely rare. Most ϕKZ-related phages had unique DNA restriction patterns, but the differences between these were only minor, and the genomes did not contain nonhomologous fragments. The spectrum of capsid polypeptides proved to be conserved in each species, and was proposed as a character necessary and sufficient for express classification of phages with an accuracy of species. Phages isolated in different geographical regions showed no substantial difference. Some phages only slightly differing in DNA restriction pattern from ϕKZ may be used to study the origin of ϕKZ genes coding for orthologs of proteins of unrelated species (other phages, pathogenic bacteria, eukaryotes). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Comparisons of the Genomes of New Giant Phages Isolated from Environmental Pseudomonas aeruginosa Strains of Different Regions

Loading next page...
 
/lp/springer_journal/comparisons-of-the-genomes-of-new-giant-phages-isolated-from-0yDqhf50wx
Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2004 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/B:RUGE.0000024972.51075.06
Publisher site
See Article on Publisher Site

Abstract

To study the genome diversity of bacteriophages from geographically distant natural populations, new giant ϕKZ-like Pseudomonas aeruginosa phages isolated in two different regions were compared with earlier known phages of three species (ϕKZ, Lin68, EL). A broad spectrum of lytic activity was demonstrated for all ϕKZ-like phages. Phages of the ϕKZ species proved to be common in natural populations of various regions, while EL- and Lin68-related phages were extremely rare. Most ϕKZ-related phages had unique DNA restriction patterns, but the differences between these were only minor, and the genomes did not contain nonhomologous fragments. The spectrum of capsid polypeptides proved to be conserved in each species, and was proposed as a character necessary and sufficient for express classification of phages with an accuracy of species. Phages isolated in different geographical regions showed no substantial difference. Some phages only slightly differing in DNA restriction pattern from ϕKZ may be used to study the origin of ϕKZ genes coding for orthologs of proteins of unrelated species (other phages, pathogenic bacteria, eukaryotes).

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 18, 2004

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off