Mammalian Genome 9, 312-315 (1998). Mammalian Genome Incorporating Noose Genn®e © Springer-Verlag New York Inc. 1998 Comparison of YACs containing mouse centromeric satellite sequences cloned in rad52 and RAD52 host strains Amanda McGuigan, Ania Manson, Morgan Haldane, Clare Huxley Department of Biochemistry and Molecular Genetics, Imperial College School of Medicine at St. Mary's, London, W2 1PG, UK Received: 14 September 1997 / Accepted: 28 November 1997 The centromeres of mammalian chromosomes are generally het- irradiation (rdMin-1) and was subsequently left out of analysis. All erochromatic and contain hundreds of kilobases of tandemly re- the others were clearly in a recombination-deficient host. peated satellite sequences. Two classes of satellite DNA, minor The size of the YACs was determined by pulsed field gel and major, have been identified at the centromeres of mouse chro- electrophoresis (PFGE) of high-molecular-weight DNA and mosomes. Minor satellite DNA consists of tandem arrays of 120- Southern blotting of four colonies for each YAC. Tables 1 and 2 bp monomers, forming uninterrupted arrays up to 500 kb long at give the sizes of the largest stable clone obtained for each YAC. the centromeres adjacent to the telomeres (Kipling et al. 1991). It Three YACs (Min-2, Min-16, and
Mammalian Genome – Springer Journals
Published: Mar 20, 2009
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