Comparison of the Effects Exerted by Luminal Ca2+ on the Sensitivity of the Cardiac Ryanodine Receptor to Caffeine and Cytosolic Ca2+

Comparison of the Effects Exerted by Luminal Ca2+ on the Sensitivity of the Cardiac Ryanodine... Ca2+ released from the sarcoplasmic reticulum (SR) via ryanodine receptor type 2 (RYR2) is the key determinant of cardiac contractility. Although activity of RYR2 channels is primary controlled by Ca2+ entry through the plasma membrane, there is growing evidence that Ca2+ in the lumen of the SR can also be effectively involved in the regulation of RYR2 channel function. In the present study, we investigated the effect of luminal Ca2+ on the response of RYR2 channels reconstituted into a planar lipid membrane to caffeine and Ca2+ added to the cytosolic side of the channel. We performed two sets of experiments when the channel was exposed to either luminal Ba2+ or Ca2+. The given ion served also as a charge carrier. Luminal Ca2+ effectively shifted the EC50 for caffeine sensitivity to a lower concentration but did not modify the response of RYR2 channels to cytosolic Ca2+. Importantly, luminal Ca2+ exerted an effect on channel gating kinetics. Both the open and closed dwell times were considerably prolonged over the whole range (response to caffeine) or the partial range (response to cytosolic Ca2+) of open probability. Our results provide strong evidence that an alteration of the gating kinetics is the result of the interaction of luminal Ca2+ with the luminally located Ca2+ regulatory sites on the RYR2 channel complex. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Comparison of the Effects Exerted by Luminal Ca2+ on the Sensitivity of the Cardiac Ryanodine Receptor to Caffeine and Cytosolic Ca2+

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Publisher
Springer-Verlag
Copyright
Copyright © 2007 by Springer Science+Business Media, Inc.
Subject
Life Sciences; Human Physiology; Biochemistry, general
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-006-7018-z
Publisher site
See Article on Publisher Site

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