Arch Virol (2003) 148: 209–219
Comparison of the amino acid sequences of RNA-dependent RNA
polymerases of cypoviruses in the family Reoviridae
, G. R. Carner
, S. W. Scott
, T. Omura
, and K. Hagiwara
Department of Entomology, Clemson University, Clemson, South Carolina, U.S.A.
Department of Plant Pathology and Physiology, Clemson University,
Clemson, South Carolina, U.S.A.
Laboratory of Virology, National Agricultural Research Center,
Tsukuba, Ibaraki, Japan
Received July 8, 2002; accepted September 19, 2002
Published online December 10, 2002
Summary. The nucleotide sequences of the genome segment S2 of Bombyx mori
cypovirus1,S2ofLymantria disparcypovirus1, S1ofLymantria dispar cypovirus
14 and S1 of a proposed new electropherotype of Trichoplusia ni cypovirus 15
were determined. These segments encoded putative RNA-dependent RNA poly-
merases(RDRPs).The deducedamino acid sequencesof RDRPs withinthe genus
Cypovirus showed 32% to 94% identities, while extent of homology between
RDRPs in the genera Cypovirus and Oryzavirus, a genus most closely related,
have originated from a common insect virus ancestor.
Viruses in the family Reoviridae have a capsid made up of concentric layers of
proteinsthat are organizedasone,twoorthreedistinct capsid shellscontaining10,
11 or 12 segments of the linear double-stranded RNA (dsRNA). The family con-
sists of nine established genera: Orthoreovirus, Orbivirus, Rotavirus, Coltivirus,
Aquareovirus, Cypovirus, Fijivirus, Phytoreovirus, and Oryzavirus  and one
recently proposed genus, Seadornavirus . Members of these genera infect a
wide range of hosts including humans, cattle, birds, ﬁsh, insects, and plants.
The nucleotide sequence data reported in this paper for BmCPV-1 S2, LdCPV-1 S2,
LdCPV-14 S1 and TnCPV-15 S1 will appear in the GenBank/EMBL/DDBJ nucleotide
sequence databases under accession numbers AF323782, AF389463, AF389452 and