Comparison of six promoters for transient expression of luciferase reporter gene in cultured Bombyx mori cells (BmN)

Comparison of six promoters for transient expression of luciferase reporter gene in cultured... The Luciferase (luc) reporter gene was used to construct recombinant plasmids containing six different promoters, which are the cytoplasmic actin3 promoter (A3), the fibroin heavy chain promoter (Fib-H), the fibroin light chain promoter (Fib-L), a glycoprotein (P25) promoter, Sericin-1 promoter (Ser-1) and the Bombyx mori nuclear polyhedrosis virus immediate (BmNPV) early protein promoter 1(IE-1), respectively. These recombinant plasmids, which are pGL3-A3-luc, pGL3-Fib-H-luc, pGL3-Fib-L-luc, pGL3-P25-luc, pGL3-Ser-1-luc and pGL3-IE-1-luc had been constructed successfully by restriction enzyme digestion and PCR analysis, and then were transfected into BmN cells to measure the activity of the six promoters to drive luc reporter gene transient expression in cells. Transfection and transcriptional experiment indicated that except pGL3-Fib-L-luc, pGL3-P25-luc, pGL3-Ser-1-luc, others three kinds of recombinant plasmids all transfected BmN cells obviously. The promoters of Fib-H, A3 and IE-1 enhanced the transient expression activity of luc reporter gene in the BmN cells and their activity strengthened sequentially. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

Comparison of six promoters for transient expression of luciferase reporter gene in cultured Bombyx mori cells (BmN)

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2012 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Animal Anatomy / Morphology / Histology; Developmental Biology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1134/S1062360412060021
Publisher site
See Article on Publisher Site

Abstract

The Luciferase (luc) reporter gene was used to construct recombinant plasmids containing six different promoters, which are the cytoplasmic actin3 promoter (A3), the fibroin heavy chain promoter (Fib-H), the fibroin light chain promoter (Fib-L), a glycoprotein (P25) promoter, Sericin-1 promoter (Ser-1) and the Bombyx mori nuclear polyhedrosis virus immediate (BmNPV) early protein promoter 1(IE-1), respectively. These recombinant plasmids, which are pGL3-A3-luc, pGL3-Fib-H-luc, pGL3-Fib-L-luc, pGL3-P25-luc, pGL3-Ser-1-luc and pGL3-IE-1-luc had been constructed successfully by restriction enzyme digestion and PCR analysis, and then were transfected into BmN cells to measure the activity of the six promoters to drive luc reporter gene transient expression in cells. Transfection and transcriptional experiment indicated that except pGL3-Fib-L-luc, pGL3-P25-luc, pGL3-Ser-1-luc, others three kinds of recombinant plasmids all transfected BmN cells obviously. The promoters of Fib-H, A3 and IE-1 enhanced the transient expression activity of luc reporter gene in the BmN cells and their activity strengthened sequentially.

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: Nov 16, 2012

References

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