ISSN 10214437, Russian Journal of Plant Physiology, 2011, Vol. 58, No. 4, pp. 703–714. © Pleiades Publishing, Ltd., 2011.
Wheatrye 2BS.2RL translocations have an
improved agronomic performance related to biotic
and/or abiotic stresses, such as Hessian fly resistance
, powdery mildew resistance, and multiple disease
resistance . Additional agronomic traits, such as
reddish glume and awn color, have also been reported
. As most of the resistance genes on 1RS, which has
been globally used for wheat improvement, have
become partially ineffective, 2BS.2RL wheatrye
translocations would be useful alternative genetic
sources for abiotic/biotic stress resistance. Moreover,
wheat chromosome 2B appears to lack any of the stor
age protein genes that affect baking quality  and can
alleviate the detrimental quality effects associated with
The Hessian fly (
(Say)) is one
of the most destructive pests of wheat (
L.) worldwide . To date, 32 major genes, desig
, conferring resistance to the Hessian
fly have been identified , and some of these genes
This text was submitted by the authors in English.
The current address: Department of Crop Sciences, University
of Illinois, Urbana, IL 61801, USA.
have been employed in cultivar improvement [2, 7].
Many Hessian fly resistance genes originated from
diploid progenitors of wheat (e.g.,
tetraploid wheat (e.g.,
, which confers resistance to the most
virulent biotype L of Hessian fly was derived from the
long arm of rye (
L., cv. Chaupon) chro
mosome 2 [2, 6].
Proteomic approaches to the 1BL.1RS transloca
tion revealed quantitative and qualitative proteomic
variations in wheat endosperm proteins . To our
knowledge, no information is available on the pro
teome of the 2BS.2RL wheatrye translocations.
Moreover, the changes of proteomes in resistant lines
under Hessian fly infestations have
not been well documented. The objectives of this study
were to identify, by proteomic approach, seed and leaf
proteins from 2BS.2RL translocations and their recur
rent parents and to compare the proteins observed in
the leaves of a Hessian fly infested and noninfested
2BS.2RL wheatrye translocation plants.
MATERIALS AND METHODS
Plant material and Hessian fly infestation.
isogenic lines (NILs) that differed by the presence or
absence of the
gene on the 2RL rye chromosome
arm were developed by backcross introgression of
Comparison of Proteomes between WheatRye Translocations
and Their Recurrent Parents
T. G. Lee
, J. W. Johnson
, and Y. W. Seo
Institute of Life Science and Natural Resources, Korea University, Seoul 136713, Republic of Korea
Department of Crop and Soil Sciences, University of Georgia, Griffin Campus, Griffin, GA 30223, USA
College of Life Sciences and Biotechnology, Korea University, Seoul 136713, Republic of Korea;
Received May 25, 2010
—2BS.2RL wheatrye translocations have presented phenotypes of improved agronomic perfor
mance, such as Hessian fly resistance. The main objective of this work was to use twodimensional electro
phoresis to identify the proteomic differences between 2BS.2RL wheatrye translocations and their recurrent
parents. The investigation of seeds revealed linespecific protein spots, such as
amylase inhibitor 0.19.
More diverse expression patterns were observed in leaves than in seeds. Protein spots found specifically in
2BS.2RL, but not in non2RL translocations were identified as
glucosidase and vacuolar ATP synthase
subunit B2, demonstrating the effects of translocated rye chromatin 2RL on common wheat genetic back
ground. When the leaf protein spots were compared in the control and Hessian flyinfested nearisogenic line
(NIL) (2BS.2RL), many downregulated proteins and specific proteins, such as
glucosidase, were detected
in the latter.
: 2BS.2RL wheatrye translocationsHessian fly, proteome analysis.
: 2DE—twodimensional electrophoresis; IEF—
isoelectric focusing; LS—Rubisco large subunit; NILs—near