Comparison of complete polyprotein sequences of two isolates of salmon alphavirus (SAV) type I and their behaviour in a salmonid cell line

Comparison of complete polyprotein sequences of two isolates of salmon alphavirus (SAV) type I... Salmon pancreas disease virus is an alphavirus (family Togaviridae ) affecting mainly Atlantic salmon ( Salmo salar L.). Both polyprotein sequences of the Scottish isolate (SAV4640) were determined and compared with those of Irish isolate SAVF93-125. High amino acid sequence similarity (99.4 %) was found. Six amino acid deletions were found in the E2 gene of SAV4640. SAVF93-125 demonstrated a high viral load in culture despite high Mx expression. Approximately 50 % of cells infected with SAVF93-125 exhibited a cytopathic effect by day 8. SAV4640 successfully entered the cells, inducing 10,500-fold higher Mx expression at day 2 compared to SAVF93-25; however, no replication was observed based on results of the nsP1 qRT-PCR. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Comparison of complete polyprotein sequences of two isolates of salmon alphavirus (SAV) type I and their behaviour in a salmonid cell line

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Publisher
Springer Journals
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1689-4
Publisher site
See Article on Publisher Site

Abstract

Salmon pancreas disease virus is an alphavirus (family Togaviridae ) affecting mainly Atlantic salmon ( Salmo salar L.). Both polyprotein sequences of the Scottish isolate (SAV4640) were determined and compared with those of Irish isolate SAVF93-125. High amino acid sequence similarity (99.4 %) was found. Six amino acid deletions were found in the E2 gene of SAV4640. SAVF93-125 demonstrated a high viral load in culture despite high Mx expression. Approximately 50 % of cells infected with SAVF93-125 exhibited a cytopathic effect by day 8. SAV4640 successfully entered the cells, inducing 10,500-fold higher Mx expression at day 2 compared to SAVF93-25; however, no replication was observed based on results of the nsP1 qRT-PCR.

Journal

Archives of VirologySpringer Journals

Published: Oct 1, 2013

References

  • Phylogenetic analyses and molecular epidemiology of European salmonid alphaviruses (SAV) based on partial E2 and nsP3 gene nucleotide sequences
    Fringuelli, E; Rowley, HM; Wilson, JC; Hunter, R; Rodger, H; Graham, DA
  • Genetic stability within the Norwegian subtype of salmonid alphavirus (family Togaviridae)
    Karlsen, M; Hodneland, K; Endresen, C; Nylund, A
  • Development of an in vitro system to measure the sensitivity to the antiviral Mx protein of fish viruses
    Lester, K; Hall, M; Urquhart, K; Gahlawat, S; Collet, B

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