Comparing metabolism during ischemia and reperfusion in free flaps of different tissue composition

Comparing metabolism during ischemia and reperfusion in free flaps of different tissue composition Using microdialysis, the interstitial kinetics of glucose, lactate, pyruvate and glycerol were studied during ischemia and reperfusion in 39 human free flaps of different tissue compositions (skin, muscle and adipose tissue). The concentration of the substances were determined repeatedly during ischemia and reperfusion, and laser doppler flowmetry was used to document revascularization. Microdialysis catheters were placed in flap tissue and similar non-operated control tissue. Increased levels of lactate and glycerol and decreased glucose and pyruvate concentrations were observed in the flaps during ischemia. We were able to document different metabolic patterns because the various flaps consisted of different tissue. Variables involved in glycolysis (glucose, lactate and pyruvate) changed faster in muscle than in other flap tissues, whereas the glycerol increase was most evident in flaps that were composed predominantly of adipose tissue. During reperfusion all metabolites returned towards pre-ischemic levels within a few hours. However, pyruvate and glucose levels sometimes remained elevated over long periods (up to 24 h of reperfusion) in muscular flaps, possibly due to hypermetabolism, increased capillary flow or other unknown factors. It can be concluded that the dynamics of metabolism vary between flaps of different tissue compositions. This can be visualized during ischemia and reperfusion by using microdialysis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Plastic Surgery Springer Journals

Comparing metabolism during ischemia and reperfusion in free flaps of different tissue composition

Loading next page...
 
/lp/springer_journal/comparing-metabolism-during-ischemia-and-reperfusion-in-free-flaps-of-xpSmZ2yz8o
Publisher
Springer-Verlag
Copyright
Copyright © 2001 by Springer-Verlag
Subject
Medicine & Public Health; Plastic Surgery
ISSN
0930-343X
eISSN
1435-0130
D.O.I.
10.1007/s002380100294
Publisher site
See Article on Publisher Site

Abstract

Using microdialysis, the interstitial kinetics of glucose, lactate, pyruvate and glycerol were studied during ischemia and reperfusion in 39 human free flaps of different tissue compositions (skin, muscle and adipose tissue). The concentration of the substances were determined repeatedly during ischemia and reperfusion, and laser doppler flowmetry was used to document revascularization. Microdialysis catheters were placed in flap tissue and similar non-operated control tissue. Increased levels of lactate and glycerol and decreased glucose and pyruvate concentrations were observed in the flaps during ischemia. We were able to document different metabolic patterns because the various flaps consisted of different tissue. Variables involved in glycolysis (glucose, lactate and pyruvate) changed faster in muscle than in other flap tissues, whereas the glycerol increase was most evident in flaps that were composed predominantly of adipose tissue. During reperfusion all metabolites returned towards pre-ischemic levels within a few hours. However, pyruvate and glucose levels sometimes remained elevated over long periods (up to 24 h of reperfusion) in muscular flaps, possibly due to hypermetabolism, increased capillary flow or other unknown factors. It can be concluded that the dynamics of metabolism vary between flaps of different tissue compositions. This can be visualized during ischemia and reperfusion by using microdialysis.

Journal

European Journal of Plastic SurgerySpringer Journals

Published: Jan 1, 2002

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off