ISSN 10214437, Russian Journal of Plant Physiology, 2012, Vol. 59, No. 3, pp. 381–388. © Pleiades Publishing, Ltd., 2012.
In arid, semiarid, tropical, and subtropical regions
of the world, high temperature has been a major limi
tation to crop productivity. High temperature has det
rimental effects on plant growth and development,
such as tassel initiation and time of flowering, pollen
sterility, and the rate and duration of endosperm cell
division . The effects of high temperature on plants
involve many physiological and biochemical changes,
including water deficit, protein denaturation and
aggregation, oxidative stress, and cellular structure
disruptions [2, 3].
Plant responses to environmental stresses have
often been associated with ROS, including
Overproduction of ROS inevitably causes
lipid peroxidation and consequently membrane injury,
protein degradation, enzyme inactivation, pigment
The text was submitted by the authors in English.
bleaching, and disruption of DNA strands [4, 5].
Because ROS are toxic but also participate in key sig
naling events, plant cells have developed different
strategies to regulate intracellular ROS concentrations
by their scavenging. Major ROSscavenging enzyme
includes superoxide dismutase (SOD), ascorbate per
oxidase (APX), catalase (CAT), and peroxidase
(POD). The balance between SOD, POD, and APX
(and/or CAT) activities in the cells is considered to be
crucial for determining the steadystate level of
. Detoxification of these compounds there
fore contributes to salt and other stress tolerance.
Dune reed (DR), which grows in the desert and
sand dune region of northwestern China, is an impor
tant ecotype of reed (
Trin.). It is
exposed frequently to a combination of stresses, such
as drought, high light, and high temperature. Under
such harsh conditions, DR vegetates and develops
normally and forms some quite large populations .
DR had proved to retain some stable variations of
morphological, physiological, and genetic character
istics in response to external stresses . It is an ideal
material for studies on the adaptations of plants to var
ious environmental conditions. In contrast, swamp
reed (SR), another ecotype of reed, grows in ponds
that are full of water all year round . It has been
Comparative Study on Calli from Two Reed Ecotypes
under Heat Stress
, Y. Jiang
, H. Zhao
, and Zh. Zhang
School of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 200235, P.R. China;
fax: +862164943128; email: SongLL713@yahoo.com.cn
StateOwned Miaoshou Forest Farm, Jingde Anhui, 242604, P.R. China
Received July 5, 2011
—The different physiological responses to heat stress in calli from two ecotypes of common reed
Trin.) plants (dune reed (DR) and swamp reed (SR)) were studied. The relative water
content, the relative growth rate, cell viability, membrane permeability (MP), H
content, MDA content,
proline level, and the activities of enzymes, such as superoxide dismutase (SOD), catalase (CAT), peroxidase
(POD), ascorbate peroxidase (APX), glutathione reductase (GR), and lipoxygenase (LOX) were assayed.
Results showed that under heat stress, DR callus could maintain the higher relative growth rate and cell via
bility than SR callus, while H
content, MDA content, and MP in SR callus increased more than in DR
callus. The activities of antioxidant enzymes, such as SOD, CAT, POD, APX, and GR in two calli were
enhanced by high temperature. However, antioxidant enzymes in DR callus showed the higher thermal sta
bility than those in SR callus. LOX activity increased more in SR callus than in DR callus under heat stress.
High temperature markedly elevated proline content in DR callus whereas had no effect on that in SR callus.
Taken together, DR callus is more thermotolerant than SR callus, which might be due to the higher activity
of antioxidant enzymes and proline level compared with SR callus under heat stress.
Keywords: Phragmites communis
, antioxidant enzymes, heat stress, reactive oxygen species, thermotolerance.
: APX—ascorbate peroxidase; CAT—catalase;
DR—dune reed; GR—glutathione reductase; LOX—lipoxygen
ase; MP—membrane permeability; NBT—nitro blue tetrazo
lium; POD—peroxidase; PVP—polyvinylpyrrolidone; RGR—
relative growth rate; RWC—relative water contents; SR—swamp
reed; SOD—superoxide dismutase; TTC—2,3,5triphenyl tetra