1062-3604/05/3601- © 2005 Pleiades Publishing, Inc.
Russian Journal of Developmental Biology, Vol. 36, No. 1, 2005, pp. 51–53. Translated from Ontogenez, Vol. 36, No. 1, 2005, pp. 61–63.
Original Russian Text Copyright © 2005 by Neznanova, Ivankov, Reunov.
The ﬁne structure of Sertoli cells was repeatedly
studied in the testes of teleostean ﬁsh. In representa-
tives of all studied ﬁsh taxa, these cells form cysts
where early spermatogenesis takes place (Guraya,
1976; Gabaeva, 1982). “Withdrawal” of Sertoli cells
from late spermatogenesis is also a common feature,
since at the spermatid stage, spermatogenic cells leave
cysts and the terminal stage of formation of the sperma-
tozoa takes place in the cavity of testes (Hurk
, 1980; Grier, 1981). It is believed that
in the postspawning gonads of teleostean ﬁsh, Sertoli
cells phagocytize nonspawned gametes and residual
bodies (Soley and Vuren, 1984; Grier
Dudois and Callard, 1989).
When considering the results of studies of the struc-
ture and functions of Sertoli cells, it becomes evident
that in all studied teleostean ﬁsh, the functions of these
cells correspond to the classical concept of Sertoli cells
of mammals and other vertebrates. Nevertheless, pre-
liminary results of histological analysis suggest that the
accessory cells of some teleostean ﬁsh differ in mor-
phology from Sertoli cells. A possible morphological
diversity of accessory cells in teleostean ﬁsh has never
been considered and deserves a special study.
In the present study, we used two species of ﬂatﬁsh,
, in which Sertoli cells are, according to the prelimi-
nary data, species speciﬁc. Application of electron
microscopy will allow a reliable conclusion about sim-
ilarity of the general morphology of these cells in the
species under comparison.
MATERIALS AND METHODS
Fishes were caught during a voyage on MRS-5005
in Peter the Great Bay, Sea of Japan, in 2002. Studies
were carried out on three ﬁshes of each species.
Small fragments of gonads were ﬁxed by 2.5% glu-
taraldehyde prepared on 0.1 M cacodylate buffer, pH
7.4, for 2 h and postﬁxed by 2% osmium tetroxide on
the same buffer. The material was then dehydrated in
ascending alcohols and acetones and embedded in
Epon-Araldite. Ultrathin sections were obtained on a
. The sec-
tions stained by uranyl acetate and lead citrate were
examined and photographed under a JEM 100B trans-
mission electron microscope (Japan).
RESULTS AND DISCUSSION
. Accessory cells forming
the follicle walls had irregular shape and many cyto-
plasmic processes embracing differentiating spermato-
genic cells. The oval nucleus was ﬁlled with dispersed
chromatin and localized in a widened compartment of
the cytoplasm. Cytoplasm was characterized by intense
vacuolization. Electron-light vacuoles were formed by
widened regions of smooth endoplasmic reticulum
. Accessory cells form-
ing the follicle walls had elongated, probably ﬂattened,
shape. The follicle wall consisted, as a rule, of several
cells contacting each other and containing nuclei of
characteristic oval-lobate shape (Fig. 1b).
Comparative Studies of Follicle Cells in Testes
S. Yu. Neznanova*, V. N. Ivankov*, and A. A. Reunov**
* Far East State University, Oktyabr’skaya ul. 27, Vladivostok, 690000 Russia
** Institute of Marine Biology, Far East Branch of the Russian Academy of Sciences,
ul. Pal’chevskogo 17, Vladivostok, 690041 Russia
Received January 9, 2004; in ﬁnal form, May 13, 2004
—Accessory cells were studied in early spermatogenesis of ﬂatﬁshes
using transmission electron microscopy. The morphological organization of acces-
sory cells in
was similar to that of Sertoli cells. In
, these cells had morphological
organization, which had not been previously described.
: ﬂatﬁsh, ultrastructure, accessory cells.