Mammalian Genome 9, 853–855 (1998). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1998 Comparative mapping of the prion gene (PRNP) locus in cattle, sheep and human with PCR-generated probes B. Castiglioni, S. Comincini, B. Drisaldi, T. Motta, L. Ferretti Istituto per la Difesa e la Valorizzazione del Germoplasma Animale, CNR, Palazzo L.I.T.A., via F.lli Cervi 93, 20090 Segrate (MI), Italy Received: 13 April 1998 / Accepted: 29 May 1998 Prions are the unconventional causative agent of sub-acute trans- All the probes were either nick-translated or directly labeled by missible spongiform encephalopathies in human and various mam- PCR in the presence of biotin 16-dUTP and digoxigenin 11-dUTP mals; for example, Scrapie of sheep, Bovine Spongiform Enceph- (Boehringer; Richard et al. 1994) with the above described prim- alopathy (BSE) of cattle, Cruetzfeldt-Jakob disease (CJD) and Fa- ers. tal Familial Insomnia (FFI) of human (Prusiner 1994). These Chromosome preparations were arranged from fibroblast cul- diseases are characterized by the accumulation in the brain tissue tures by standard procedures, except for the hypotonic treatment, Sc of a prion protein, PrP that is a partially proteinase-resistant 0.02 M KCl, 37°C, 13 min (bovine and ovine). After a few days at isoform of the
Mammalian Genome – Springer Journals
Published: Oct 1, 1998
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