Comparative mapping of the prion gene (PRNP) locus in cattle, sheep and human with PCR-generated probes

Comparative mapping of the prion gene (PRNP) locus in cattle, sheep and human with PCR-generated... Mammalian Genome 9, 853–855 (1998). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1998 Comparative mapping of the prion gene (PRNP) locus in cattle, sheep and human with PCR-generated probes B. Castiglioni, S. Comincini, B. Drisaldi, T. Motta, L. Ferretti Istituto per la Difesa e la Valorizzazione del Germoplasma Animale, CNR, Palazzo L.I.T.A., via F.lli Cervi 93, 20090 Segrate (MI), Italy Received: 13 April 1998 / Accepted: 29 May 1998 Prions are the unconventional causative agent of sub-acute trans- All the probes were either nick-translated or directly labeled by missible spongiform encephalopathies in human and various mam- PCR in the presence of biotin 16-dUTP and digoxigenin 11-dUTP mals; for example, Scrapie of sheep, Bovine Spongiform Enceph- (Boehringer; Richard et al. 1994) with the above described prim- alopathy (BSE) of cattle, Cruetzfeldt-Jakob disease (CJD) and Fa- ers. tal Familial Insomnia (FFI) of human (Prusiner 1994). These Chromosome preparations were arranged from fibroblast cul- diseases are characterized by the accumulation in the brain tissue tures by standard procedures, except for the hypotonic treatment, Sc of a prion protein, PrP that is a partially proteinase-resistant 0.02 M KCl, 37°C, 13 min (bovine and ovine). After a few days at isoform of the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Comparative mapping of the prion gene (PRNP) locus in cattle, sheep and human with PCR-generated probes

Loading next page...
 
/lp/springer_journal/comparative-mapping-of-the-prion-gene-prnp-locus-in-cattle-sheep-and-N0DI8t990L
Publisher
Springer-Verlag
Copyright
Copyright © 1998 by Springer-Verlag New York Inc.
Subject
Life Sciences; Cell Biology; Animal Genetics and Genomics; Human Genetics
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003359900882
Publisher site
See Article on Publisher Site

Abstract

Mammalian Genome 9, 853–855 (1998). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1998 Comparative mapping of the prion gene (PRNP) locus in cattle, sheep and human with PCR-generated probes B. Castiglioni, S. Comincini, B. Drisaldi, T. Motta, L. Ferretti Istituto per la Difesa e la Valorizzazione del Germoplasma Animale, CNR, Palazzo L.I.T.A., via F.lli Cervi 93, 20090 Segrate (MI), Italy Received: 13 April 1998 / Accepted: 29 May 1998 Prions are the unconventional causative agent of sub-acute trans- All the probes were either nick-translated or directly labeled by missible spongiform encephalopathies in human and various mam- PCR in the presence of biotin 16-dUTP and digoxigenin 11-dUTP mals; for example, Scrapie of sheep, Bovine Spongiform Enceph- (Boehringer; Richard et al. 1994) with the above described prim- alopathy (BSE) of cattle, Cruetzfeldt-Jakob disease (CJD) and Fa- ers. tal Familial Insomnia (FFI) of human (Prusiner 1994). These Chromosome preparations were arranged from fibroblast cul- diseases are characterized by the accumulation in the brain tissue tures by standard procedures, except for the hypotonic treatment, Sc of a prion protein, PrP that is a partially proteinase-resistant 0.02 M KCl, 37°C, 13 min (bovine and ovine). After a few days at isoform of the

Journal

Mammalian GenomeSpringer Journals

Published: Oct 1, 1998

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off