Oral Cancer (2018) 2:37–44
Comparative histomorphometric study of intraepithelial papillary
capillaries on leukoplakia with/without dierent dysplastic grading
and squamous cell carcinoma of the oral mucosa
· Hiroyuki Sakamaki
· Masahiro Higuchi
· Masaaki Suemitsu
· Chieko Taguchi
· Ko Ito
· Tadahiko Utsunomiya
· Toshirou Kondoh
· Kayo Kuyama
Received: 17 December 2017 / Accepted: 5 March 2018 / Published online: 12 March 2018
© Springer International Publishing AG, part of Springer Nature 2018
Purpose Tumor growth is closely related to the development of an intrinsic vascular network. However, microvascular
appearance was not regarding highly to pathological diagnosis, though clinical applicability of endoscopic narrow-bad imag-
ing (NBI) is developed in the oral cavity. To analyze changes of intraepithelial papillary capillary loop (IPCLs) patterns as
the utility for pathological diagnosis, comparative histomorphometric analysis of IPCLs among squamous cell hyperplasia,
leukoplakia with low- or high-grade dysplasia, and oral squamous cell carcinoma (OSCC) had performed.
Methods Subjects were 40 clinically hyperkeratosis, histologically consisted 5 groups; each 10 of squamous cell hyperplasia,
low- or high-grade dysplasia, OSCC and normal mucosae. Sections with smooth muscle actin and CD34 immunoreactivi-
ties were used to examine comparative histomorphometric analysis on the basis of IPCL irregularities. The area, shortest
diameter, proximity to the basement membrane and circularity were measured using Win Roof image analysis software.
Statistical analyses were done by Kruskal–Wallis and Steel–Dwass tests. Values of p < 0.05 were considered signiﬁcant.
Results Larger vessel diameter/areas, more extension and smaller circularity with dysplastic grading, and OSCC showed the
most prominent of them. In short, the changes in vessels of IPCLs in high-grade dysplasia and OSCC involve two factors:
increased vessel diameter and prolongation of IPCLs toward the surface.
Conclusions The microvascular irregularity and proliferation composed by IPCLs and IPCL like abnormal capillaries were
observed pathologically in squamous epithelial lesions of the oral cavity. The change of IPCLs patterns can be of help in
detecting for high-grade dysplasia or OSCC.
Keywords Oral leukoplakia · Hyperkeratosis · Epithelial dysplasia · IPCL · Histomorphometric analysis
* Kayo Kuyama
Department of Oral Pathology, Nihon University School
of Dentistry at Matsudo, Matsudo, Japan
Department of Maxillofacial Surgery, Nihon University
School of Dentistry at Matsudo, Matsudo, Japan
Department of Dental Hygiene, Faculty of Health Care
Sciences, Chiba Prefectural University of Health Sciences,
Department of Preventive and Public Oral Health, Nihon
University School of Dentistry at Matsudo, Matsudo, Japan