Comparative analysis of five varieties in Perilla frutescens (L.) Britton by 45S rDNA FISH and 5S rDNA sequences

Comparative analysis of five varieties in Perilla frutescens (L.) Britton by 45S rDNA FISH and 5S... In this study, the relationships of five varieties in Perilla frutescens (L.) Britton were analyzed. All the varieties showed the same chromosome number 2n = 40, and the banding patterns of PI-DAPI staining and the FISH results of 45S rDNA probe were on the same site of the chromosome pair. The 5S rRNA gene spacers were ranged from 413 bp in P. frutescens var. purpurascens and 408 bp in var. frutescens. The varieties analyzed here were clustered into two clades according to the phylogenetic analysis. The sequence identity of 96% between var. auriculato-dentata and var. arguta suggested they could be considered the same variety. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Comparative analysis of five varieties in Perilla frutescens (L.) Britton by 45S rDNA FISH and 5S rDNA sequences

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Publisher
Springer Journals
Copyright
Copyright © 2009 by Pleiades Publishing, Ltd.
Subject
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795409040085
Publisher site
See Article on Publisher Site

Abstract

In this study, the relationships of five varieties in Perilla frutescens (L.) Britton were analyzed. All the varieties showed the same chromosome number 2n = 40, and the banding patterns of PI-DAPI staining and the FISH results of 45S rDNA probe were on the same site of the chromosome pair. The 5S rRNA gene spacers were ranged from 413 bp in P. frutescens var. purpurascens and 408 bp in var. frutescens. The varieties analyzed here were clustered into two clades according to the phylogenetic analysis. The sequence identity of 96% between var. auriculato-dentata and var. arguta suggested they could be considered the same variety.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Apr 25, 2009

References

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