Comparative analysis of antibody induction and protection against influenza virus infection by DNA immunization with HA, HAe, and HA1 in mice

Comparative analysis of antibody induction and protection against influenza virus infection by... Plasmid DNA vaccines are considered alternatives to inactivated influenza virus vaccines to control influenza. Vaccination with a hemagglutinin (HA)-, HA ectodomain (HAe)-, or HA subunit 1 (HA1)-based vaccine can stimulate protective immunity in animals. The aim of this study was to compare their capacity to induce an antibody response and protection against influenza virus infection in mice after DNA vaccination. We constructed three expression vectors encoding full-length HA, HAe, or HA1 of the A/California/07/2009 influenza A virus and designed three animal experiments: (i) BALB/c mice were immunized twice with 30 μg of the HA, HAe, or HA1 DNA vaccine with high-voltage electroporation (100 V), and 3 weeks after boosting, they were challenged with a lethal dose of virus. (ii) Immunization and challenge were as in experiment i, but with low-voltage electroporation (10 V). (iii) Mice were immunized once with 50 μg of DNA and challenged 1 week later. The immunogenic effects of the three DNA vaccines were evaluated in terms of antibody titer, survival rate, bodyweight change, and lung viral titer. In all three experiments, both HA and HAe induced higher antibody and neutralization titers than HA1. Following challenge with a lethal mouse-adapted homologous virus, both HA and HAe reduced the viral titers in lung washes or offered better protection from weight loss than HA1 in experiments ii and iii. Thus, HA1 induces a lower immune response than HA or HAe when used as a DNA vaccination. Our data should be valuable in choosing the optimal candidate vaccine when faced with the threat of pandemic influenza. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Comparative analysis of antibody induction and protection against influenza virus infection by DNA immunization with HA, HAe, and HA1 in mice

Loading next page...
 
/lp/springer_journal/comparative-analysis-of-antibody-induction-and-protection-against-todAxaYhif
Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1878-1
Publisher site
See Article on Publisher Site

References

  • Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic
    Smith, GJ; Vijaykrishna, D; Bahl, J; Lycett, SJ; Worobey, M; Pybus, OG; Ma, SK; Cheung, CL; Raghwani, J; Bhatt, S; Peiris, JS; Guan, Y; Rambaut, A
  • In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses
    Itoh, Y; Shinya, K; Kiso, M; Watanabe, T; Sakoda, Y; Hatta, M; Muramoto, Y; Tamura, D; Sakai-Tagawa, Y; Noda, T; Sakabe, S; Imai, M; Hatta, Y; Watanabe, S; Li, C; Yamada, S; Fujii, K; Murakami, S; Imai, H; Kakugawa, S; Ito, M; Takano, R; Iwatsuki-Horimoto, K; Shimojima, M; Horimoto, T; Goto, H; Takahashi, K; Makino, A; Ishigaki, H; Nakayama, M; Okamatsu, M; Warshauer, D; Shult, PA; Saito, R; Suzuki, H; Furuta, Y; Yamashita, M; Mitamura, K; Nakano, K; Nakamura, M; Brockman-Schneider, R; Mitamura, H; Yamazaki, M; Sugaya, N; Suresh, M; Ozawa, M; Neumann, G; Gern, J; Kida, H; Ogasawara, K; Kawaoka, Y
  • Comparing the ability of a series of viral protein-expressing plasmid DNAs to protect against H5N1 influenza virus
    Chen, Q; Kuang, H; Wang, H; Fang, F; Yang, Z; Zhang, Z; Zhang, X; Chen, Z
  • Protection of chickens against avian influenza with nonreplicating adenovirus-vectored vaccine
    Toro, H; Tang, DC
  • Gene transfer into muscle by electroporation in vivo
    Aihara, H; Miyazaki, J
  • Protection against avian influenza H9N2 virus challenge by immunization with hemagglutinin- or neuraminidase-expressing DNA in BALB/c mice
    Qiu, M; Fang, F; Chen, Y; Wang, H; Chen, Q; Chang, H; Wang, F; Zhang, R; Chen, Z
  • DNA vaccines: immunology, application, and optimization
    Gurunathan, S; Klinman, DM; Seder, RA

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial