*Sequence data reported in this report have been deposited in the GenBank data library
under accession numbers U62149 (ALA VP4), U62150 (C-11 VP4), U62151 (B-2 VP4),
U62152 (BAP-2 VP4), and U 62153 (BAP-2 VP7).
Arch Virol (1997) 142: 1059—1069
Comparative amino acid sequence analysis of the outer capsid protein
VP4 from four lapine rotavirus strains reveals identity with
genotype P human rotaviruses*
, M. K. Estes
, and M. E Conner
Division of Molecular Virology, Baylor College of Medicine, Houston, Texas, U.S.A.
VA Medical Center, Houston, Texas, U.S.A.
Accepted October 2, 1996
Summary. The genes encoding the outer capsid VP4 proteins of four lapine
rotavirus strains, three isolated in the US (ALA, C-11 and BAP-2) and one
isolated in Japan (R-2) were sequenced, and the predicted amino acid (aa)
sequence was compared to all known rotavirus genotypes. A high degree of aa
identity (96.8—98.9%) was found among the American lapine strains, while the
Japanese rotavirus strain R-2 shared less aa identity (89.5—90.0%) with the
American strains. The four lapine rotaviruses shared the closest aa identity
(90.6—94.9%) with the P genotype, consisting of viruses isolated from
humans in Italy, Finland and Thailand. These results indicate that the VP4
protein of the four lapine strains are genotype P14, and that among lapine strains
there are possibly two subtypes, one represented by the American lapine strains
and the other by the Japanese R-2 strain.
Rotaviruses are the major pathogens associated with viral gastroenteritis in
humans. Rotaviruses belong to the Reoviridae family and are characterized by
a genome consisting of 11 segments of double-stranded RNA, enclosed in
a triple-layered protein capsid (reviewed in ). The two outer capsid proteins of
group A rotaviruses, VP4 and VP7, independently elicit neutralizing antibodies
and induce protective immunity [16, 17, 29]. Rotavirus infectivity is enhanced by
proteolytic cleavage of VP4 into VP8* and VP5* and VP4 has been associated