Commentary: phenotypic screening of radiation hybrid panels

Commentary: phenotypic screening of radiation hybrid panels Review Incorporating Mouse Genome Mammalian Genome 12, 879–881 (2001). © Springer-Verlag New York Inc. 2001 DOI: 10.1007/s00335-001-4999-x Susan R. Ross Department of Microbiology/Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA Received: 6 August 2001 / Accepted: 28 August 2001 One of the major tools used in generating high-resolution maps is types, this method could be used for virtually any virus whose radiation hybrid (RH) panels made from cells of the organism of envelope or surface protein was stably expressed on particles that interest and Chinese hamster fibroblasts. Such panels have been had encapsidated genomes with either selectable (i.e., neomycin used extensively for a number of species and have resulted in maps resistance) or phenotypic (i.e., b-galactosidase, alkaline phospha- that integrate genetic, physical, and expressed sequence tag (EST) tase, or, more recently, green fluorescent protein) markers (Fig. 1). data. Typically, such RH panels are screened with known genes, However, the mapping data obtained from screening somatic cell ESTs, and other markers by polymerase chain reaction (PCR) hybrids, while useful in terms of chromosomal placement of the to assign chromosomal location. This has led to the placement gene, was not sufficiently precise to allow gene identification. This of >30,000 markers Mammalian Genome Springer Journals

Commentary: phenotypic screening of radiation hybrid panels

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Copyright © 2001 by Springer-Verlag New York Inc.
Life Sciences; Cell Biology; Anatomy; Zoology
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