Co-production of lipids, eicosapentaenoic acid, fucoxanthin, and chrysolaminarin by Phaeodactylum tricornutum cultured in a flat-plate photobioreactor under varying nitrogen conditions

Co-production of lipids, eicosapentaenoic acid, fucoxanthin, and chrysolaminarin by Phaeodactylum... The marine diatom Phaeodactylum tricornutum is a polymorphological, ecologically significant, and well-studied model of unicellular microalga. This diatom can accumulate diverse important metabolites. Herein, we cultured P. tricornutum in an internally installed tie-piece flat-plate photobioreactor under 14.5 m mol L−1 (high nitrogen, HN) and 2.9 m mol L−1 (low nitrogen, LN) of KNO3 and assessed its time-resolved changes in biochemical compositions. The results showed that HN was inductive to accumulate high biomass (4.1 g L−1). However, the LN condition could accelerate lipid accumulation in P. tricornutum. The maximum total lipid (TL) content under LN was up to 42.5% of biomass on day 12. Finally, neutral lipids (NLs) were 63.8% and 75.7% of TLs under HN and LN, respectively. The content of EPA ranged from 2.3% to 1.5% of dry weight during the growth period under the two culture conditions. Peak volumetric lipid productivity of 128.4 mg L−1d−1 was achieved in the HN group (on day 9). The highest volumetric productivity values of EPA, chrysolaminarin, and fucoxanthin were obtained in the exponential phase (on day 6) under HN, which were 9.6, 93.6, and 4.7 mg L−1d−1, respectively. In conclusion, extractable amounts of lipids, EPA, fucoxanthin, and chrysolaminarin could be obtained from P. tricornutum by regulating the culture conditions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Ocean University of China Springer Journals

Co-production of lipids, eicosapentaenoic acid, fucoxanthin, and chrysolaminarin by Phaeodactylum tricornutum cultured in a flat-plate photobioreactor under varying nitrogen conditions

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Publisher
Science Press
Copyright
Copyright © 2017 by Science Press, Ocean University of China and Springer-Verlag GmbH Germany
Subject
Earth Sciences; Oceanography; Meteorology
ISSN
1672-5182
eISSN
1993-5021
D.O.I.
10.1007/s11802-017-3174-2
Publisher site
See Article on Publisher Site

Abstract

The marine diatom Phaeodactylum tricornutum is a polymorphological, ecologically significant, and well-studied model of unicellular microalga. This diatom can accumulate diverse important metabolites. Herein, we cultured P. tricornutum in an internally installed tie-piece flat-plate photobioreactor under 14.5 m mol L−1 (high nitrogen, HN) and 2.9 m mol L−1 (low nitrogen, LN) of KNO3 and assessed its time-resolved changes in biochemical compositions. The results showed that HN was inductive to accumulate high biomass (4.1 g L−1). However, the LN condition could accelerate lipid accumulation in P. tricornutum. The maximum total lipid (TL) content under LN was up to 42.5% of biomass on day 12. Finally, neutral lipids (NLs) were 63.8% and 75.7% of TLs under HN and LN, respectively. The content of EPA ranged from 2.3% to 1.5% of dry weight during the growth period under the two culture conditions. Peak volumetric lipid productivity of 128.4 mg L−1d−1 was achieved in the HN group (on day 9). The highest volumetric productivity values of EPA, chrysolaminarin, and fucoxanthin were obtained in the exponential phase (on day 6) under HN, which were 9.6, 93.6, and 4.7 mg L−1d−1, respectively. In conclusion, extractable amounts of lipids, EPA, fucoxanthin, and chrysolaminarin could be obtained from P. tricornutum by regulating the culture conditions.

Journal

Journal of Ocean University of ChinaSpringer Journals

Published: Aug 12, 2017

References

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