Cloning, sequencing, and promoter identification of Blueberry red ringspot virus , a member of the family Caulimoviridae with similarities to the “Soybean chlorotic mottle-like” genus

Cloning, sequencing, and promoter identification of Blueberry red ringspot virus , a member of... The double-stranded DNA genome of Blueberry red ringspot virus (BRRV), a member of the family Caulimoviridae , was cloned and sequenced. The genome organization and relationships of the 8303 nt sequence revealed BRRV to be a tentative member of the genus that has been provisionally named “Soybean chlorotic mottle-like viruses”, rather than a member of the genus Caulimovirus , in which it had been placed previously. Insertion of the putative 35S promoter homolog of BRRV into promoterless constructs carrying the UidA (β-glucuronidase) gene resulted in high-level transient expression from cranberry and stable expression from transgenic tobacco. Sequences of 5′-RACE clones derived from transcripts from transgenic tobacco were consistent with the map position of the promoter. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Cloning, sequencing, and promoter identification of Blueberry red ringspot virus , a member of the family Caulimoviridae with similarities to the “Soybean chlorotic mottle-like” genus

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Publisher
Springer-Verlag
Copyright
Copyright © 2002 by Springer-Verlag/Wien
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-002-0866-7
Publisher site
See Article on Publisher Site

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