Cloning of two plant cDNAs encoding a β-type proteasome subunit and a transformer-2-like SR-related protein: early induction of the corresponding genes in tobacco cells treated with cryptogein

Cloning of two plant cDNAs encoding a β-type proteasome subunit and a transformer-2-like... We report the successful combination of mRNA differential-display reverse-transcription PCR (DDRT-PCR) and 5′-rapid amplification of cDNA ends (5′-RACE) in order to isolate full-length cDNAs corresponding to genes activated in tobacco cells treated with cryptogein within 60 min. Cloning and sequencing of two cDNAs, called ‘tcI 7’ and ‘tcI 14’ (for tobacco cryptogein-induced), allowed the identification of open reading frames. Deduced amino-acid sequences of ‘tcI 7’ and ‘tcI 14’ showed significant homologies with a β-type proteasome subunit and a transformer-2-like serine/arginine-rich (SR) ribonucleoprotein, respectively. The accumulation of mRNAs corresponding to ‘tcI 7’ started 30 min after the addition of cryptogein to tobacco cell suspensions and continued up to 180 min, whereas the accumulation of ‘tcI 14’ corresponding mRNAs was transitory between 30 and 60 min. These results indicated a transcriptional activation of the corresponding genes early after elicitation of tobacco cells by cryptogein. The biological significance of this activation remains to be elucidated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Cloning of two plant cDNAs encoding a β-type proteasome subunit and a transformer-2-like SR-related protein: early induction of the corresponding genes in tobacco cells treated with cryptogein

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 1997 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1005833216479
Publisher site
See Article on Publisher Site

Abstract

We report the successful combination of mRNA differential-display reverse-transcription PCR (DDRT-PCR) and 5′-rapid amplification of cDNA ends (5′-RACE) in order to isolate full-length cDNAs corresponding to genes activated in tobacco cells treated with cryptogein within 60 min. Cloning and sequencing of two cDNAs, called ‘tcI 7’ and ‘tcI 14’ (for tobacco cryptogein-induced), allowed the identification of open reading frames. Deduced amino-acid sequences of ‘tcI 7’ and ‘tcI 14’ showed significant homologies with a β-type proteasome subunit and a transformer-2-like serine/arginine-rich (SR) ribonucleoprotein, respectively. The accumulation of mRNAs corresponding to ‘tcI 7’ started 30 min after the addition of cryptogein to tobacco cell suspensions and continued up to 180 min, whereas the accumulation of ‘tcI 14’ corresponding mRNAs was transitory between 30 and 60 min. These results indicated a transcriptional activation of the corresponding genes early after elicitation of tobacco cells by cryptogein. The biological significance of this activation remains to be elucidated.

Journal

Plant Molecular BiologySpringer Journals

Published: Sep 30, 2004

References

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