Cloning, expression, and characterization of phyA gene from Ipomoea batatas

Cloning, expression, and characterization of phyA gene from Ipomoea batatas Phytochrome A (phyA), as a photoreceptor, has a profound influence on plant growth and development and participates in many important physiological processes, such as seed germination, seeding deetiolation, and flowering. On the basis of transcriptome database of sweet potato (Ipomoea batatas (L.) Lam.), phyA gene was cloned and sequenced. The gene has an open reading frame (ORF) of 3384 bp, encodes a protein with 1127 amino acid residues and a mol wt of 125.03 kD. More interestingly, it was found that there were three highly homologous phyA cDNA isoforms in sweet potato. In addition, when the genomic sequence of phyA gene was obtained by PCR using sweet potato genomic DNA as a template, it contained 4 exons and 3 introns with a total size of 4957 bp. The cDNA of phyA gene was inserted into the vector pET-32a(+) and successfully expressed in Escherichia coli BL21(DE3). The results of quantitative real-time PCR indicated that the expression level of phyA gene was higher in stem, followed by leaf, and the lowest in tuberous root. Besides, the expression of phyA mRNA in the mature leaf showed an obvious circadian rhythm in a day: mRNA accumulation gradually increased in darkness and decreased in the daylight. Russian Journal of Plant Physiology Springer Journals

Cloning, expression, and characterization of phyA gene from Ipomoea batatas

Loading next page...
Pleiades Publishing
Copyright © 2015 by Pleiades Publishing, Ltd.
Life Sciences; Plant Physiology; Plant Sciences
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial