Mammalian Genome 8, 863-865 (1997). jtL e//o///e 9 Springer-Verlag New York Inc. 1997 Cloning, chromosomal mapping, and expression of the human eHAND gene Mark W. Russell, 1 Priscilla Baker, z Seigo Izumo 3 1Cardiovascular Research Center and Departments of Pediatrics and Communicable Diseases, F1310 MCHC/Box 0204, University of Michigan Medical Center, 1500 East Medical Center Dr., Ann Arbor, Michigan 48109-0204, USA 2National Center for Human Genome Research, National Institutes of Health, Bethesda, Maryland, USA 3Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan, USA Received: 15 April 1997 / Accepted: 20 June 1997 Basic helix-loop-helix transcription factors (bHLH) have been and one 1.6 kb, were isolated by digestion with EcoRI, subcloned demonstrated to be vital for skeletal muscle, neuronal, hematopoi- into a pBluescript vector, and sequenced on both strands by fluo- etic, craniofacial, and limb development (Jan and Jan 1993; rescent automated sequencing (Model 377A, Applied Biosystems, Zhuang et al. 1994; Howard et al. 1996). Recently, two bHLH Foster City, Calif.). transcription factors, dHAND and eHAND, were determined to be The resulting 1.62-kb human eHAND cDNA sequence (Gen- required for normal cardiac development in both the mouse and bank accession: pending) includes a 190-bp 5' untranslated
Mammalian Genome – Springer Journals
Published: Mar 24, 2009
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