Cloning and functional characterization of a fatty acid Δ6-desaturase from Oenothera biennis: Production of γ-linolenic acid by heterologous expression in Saccharomyces cerevisiae

Cloning and functional characterization of a fatty acid Δ6-desaturase from Oenothera biennis:... Evening primrose Oenothera biennis is grown commercially for γ-linolenic acid (GLA) production by the fatty acid Δ6-desaturase enzymatic reaction. Based on available sequence information, the O. biennis fatty acid Δ6-desaturase gene was cloned, using rapid amplification of cDNA ends methods and reverse transcription-polymerase chain reaction. The deduced amino acid sequence showed high similarity to the plant Δ6-desaturases, which comprised the characteristics of membrane-bound desaturases, including three conserved histidine-rich motifs, hydropathy profile, and a cytochrome b5 domain at the N-terminus. Heterologous expression of the coding sequence in Saccharomyces cerevisiae resulted in the synthesis and accumulation of GLA when provided with linoleic acid substrate. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Cloning and functional characterization of a fatty acid Δ6-desaturase from Oenothera biennis: Production of γ-linolenic acid by heterologous expression in Saccharomyces cerevisiae

Loading next page...
 
/lp/springer_journal/cloning-and-functional-characterization-of-a-fatty-acid-6-desaturase-2io9vQ3ZFn
Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Sciences ; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443710040163
Publisher site
See Article on Publisher Site

Abstract

Evening primrose Oenothera biennis is grown commercially for γ-linolenic acid (GLA) production by the fatty acid Δ6-desaturase enzymatic reaction. Based on available sequence information, the O. biennis fatty acid Δ6-desaturase gene was cloned, using rapid amplification of cDNA ends methods and reverse transcription-polymerase chain reaction. The deduced amino acid sequence showed high similarity to the plant Δ6-desaturases, which comprised the characteristics of membrane-bound desaturases, including three conserved histidine-rich motifs, hydropathy profile, and a cytochrome b5 domain at the N-terminus. Heterologous expression of the coding sequence in Saccharomyces cerevisiae resulted in the synthesis and accumulation of GLA when provided with linoleic acid substrate.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jul 7, 2010

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off