Cloning and Expression of the Mycoplasma hominis ftsZ Gene for a Cell Division Protein

Cloning and Expression of the Mycoplasma hominis ftsZ Gene for a Cell Division Protein A Mycoplasma hominis chromosomal fragment containing the full-length ftsZ gene was cloned and sequenced. Natural expression of this gene was demonstrated by reverse transcription–polymerase chain reaction (RT–PCR) with total RNA. TheM. hominis FtsZ protein was shown to differ substantially from its counterparts of two otherMycoplasma species, M. genitalium,and M. pneumoniae.The possibility of M. hominis ftsZ expression in Escherichia coliwas demonstrated with several bacterial strains. The M. hominis FtsZ protein was isolated from E. coli cells transformed with recombinant plasmids carrying the M. hominis ftsZ gene. Complementation between theE. coli and M. hominis FtsZ proteins was observed in transformants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Cloning and Expression of the Mycoplasma hominis ftsZ Gene for a Cell Division Protein

Loading next page...
 
/lp/springer_journal/cloning-and-expression-of-the-mycoplasma-hominis-ftsz-gene-for-a-cell-uWS7kZ2heF
Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2003 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1023252507481
Publisher site
See Article on Publisher Site

Abstract

A Mycoplasma hominis chromosomal fragment containing the full-length ftsZ gene was cloned and sequenced. Natural expression of this gene was demonstrated by reverse transcription–polymerase chain reaction (RT–PCR) with total RNA. TheM. hominis FtsZ protein was shown to differ substantially from its counterparts of two otherMycoplasma species, M. genitalium,and M. pneumoniae.The possibility of M. hominis ftsZ expression in Escherichia coliwas demonstrated with several bacterial strains. The M. hominis FtsZ protein was isolated from E. coli cells transformed with recombinant plasmids carrying the M. hominis ftsZ gene. Complementation between theE. coli and M. hominis FtsZ proteins was observed in transformants.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 7, 2004

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off