Cloning and Expression of the Mycoplasma hominis ftsZ Gene for a Cell Division Protein

Cloning and Expression of the Mycoplasma hominis ftsZ Gene for a Cell Division Protein A Mycoplasma hominis chromosomal fragment containing the full-length ftsZ gene was cloned and sequenced. Natural expression of this gene was demonstrated by reverse transcription–polymerase chain reaction (RT–PCR) with total RNA. TheM. hominis FtsZ protein was shown to differ substantially from its counterparts of two otherMycoplasma species, M. genitalium,and M. pneumoniae.The possibility of M. hominis ftsZ expression in Escherichia coliwas demonstrated with several bacterial strains. The M. hominis FtsZ protein was isolated from E. coli cells transformed with recombinant plasmids carrying the M. hominis ftsZ gene. Complementation between theE. coli and M. hominis FtsZ proteins was observed in transformants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Cloning and Expression of the Mycoplasma hominis ftsZ Gene for a Cell Division Protein

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2003 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1023252507481
Publisher site
See Article on Publisher Site

Abstract

A Mycoplasma hominis chromosomal fragment containing the full-length ftsZ gene was cloned and sequenced. Natural expression of this gene was demonstrated by reverse transcription–polymerase chain reaction (RT–PCR) with total RNA. TheM. hominis FtsZ protein was shown to differ substantially from its counterparts of two otherMycoplasma species, M. genitalium,and M. pneumoniae.The possibility of M. hominis ftsZ expression in Escherichia coliwas demonstrated with several bacterial strains. The M. hominis FtsZ protein was isolated from E. coli cells transformed with recombinant plasmids carrying the M. hominis ftsZ gene. Complementation between theE. coli and M. hominis FtsZ proteins was observed in transformants.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 7, 2004

References

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