Cloning and expression analysis of four DELLA genes in peanut

Cloning and expression analysis of four DELLA genes in peanut Peanut (Arachis hypogaea L.), as a source of oil and protein, is the second-most important grain legume cultivated in the world. Peanut is a relatively drought-tolerant crop; however, the molecular biology research of peanut is far behind other crops; the molecular mechanisms of its stress resistance are poorly understood. DELLA proteins, negative regulators of gibberellin signaling pathway in plants, promote survival of plants in adverse environments. In this study, four DELLA homologue genes were isolated using peanut transcriptome sequences. Molecular phylogenetic analysis revealed that these four AhDELLAs fall into three distinct groups: AhDELLA1 and AhDELLA2 clustered into two distinct groups, while AhDELLA3 and AhDELLA4 were in one group, which was separated from other DELLAs. qRT-PCR results showed that these four DELLA genes were expressed differentially in various peanut tissues. The AhDELLA1 and AhDELLA2 genes were expressed ubiquitously in different tissues. AhDELLA3 and AhDELLA4 showed much higher expression level in flowers and seeds as compared with other organs. The expression of four AhDELLA genes was temporally induced by PEG 6000 treatment. The AhDELLA3 and AhDELLA4 transcripts were significantly induced by NaCl treatment, while the expression of AhDELLA1 and AhDELLA2 did not change much under salt stress. The possible role of DELLA proteins in peanut development and responses to abiotic stresses is discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Cloning and expression analysis of four DELLA genes in peanut

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Publisher
Pleiades Publishing
Copyright
Copyright © 2015 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443715010021
Publisher site
See Article on Publisher Site

Abstract

Peanut (Arachis hypogaea L.), as a source of oil and protein, is the second-most important grain legume cultivated in the world. Peanut is a relatively drought-tolerant crop; however, the molecular biology research of peanut is far behind other crops; the molecular mechanisms of its stress resistance are poorly understood. DELLA proteins, negative regulators of gibberellin signaling pathway in plants, promote survival of plants in adverse environments. In this study, four DELLA homologue genes were isolated using peanut transcriptome sequences. Molecular phylogenetic analysis revealed that these four AhDELLAs fall into three distinct groups: AhDELLA1 and AhDELLA2 clustered into two distinct groups, while AhDELLA3 and AhDELLA4 were in one group, which was separated from other DELLAs. qRT-PCR results showed that these four DELLA genes were expressed differentially in various peanut tissues. The AhDELLA1 and AhDELLA2 genes were expressed ubiquitously in different tissues. AhDELLA3 and AhDELLA4 showed much higher expression level in flowers and seeds as compared with other organs. The expression of four AhDELLA genes was temporally induced by PEG 6000 treatment. The AhDELLA3 and AhDELLA4 transcripts were significantly induced by NaCl treatment, while the expression of AhDELLA1 and AhDELLA2 did not change much under salt stress. The possible role of DELLA proteins in peanut development and responses to abiotic stresses is discussed.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jan 4, 2015

References

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