Mammalian Genome 9, 316-319 (1998). Mammalian Genome Incorporating Mouse Genome m Springer-Verlag New York Inc. 1998 Cloning and chromosomal localization of mouse keratocan, a corneal keratan sulfate proteoglycan Jane R. Dunlevy,' Shukti Chakravarti, 2 Phurba Gyalzen, 2 Jean-Paul Vergnes, 3 John R. Hassell' 'Shriners Hospital for Children-Tampa and University of South Florida, College of Medicine, 12502 North Pine Drive, Tampa, Florida 33612-9499, USA 'Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA 3 Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA Received: 5 September 1997 / Accepted: 26 November 1997 The corneal stroma consists of a highly structured extracellular gation analysis of restriction fragment length variants (RFLVs) in matrix that is maintained by specialized fibroblasts called kerato- recombinant inbred (RI) strains of mice. cytes. The transparency of the cornea is dependent on the presence Total RNA was isolated from mouse whole eyes by use of a of highly sulfated proteoglycans (PG) that can bind collagen and guanidinium thiocyanate-phenol-chloroform procedure (Chomc- can regulate collagen fibril diameter (Rada et al. 1993). The cor- zynski and Sacchi 1987). An mRNA purification kit (Pharmacia neal proteoglycans that have been identified contain numerous Biotech)
Mammalian Genome – Springer Journals
Published: Mar 20, 2009
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