ISSN 1021-4437, Russian Journal of Plant Physiology, 2009, Vol. 56, No. 1, pp. 118–125. © Pleiades Publishing, Ltd., 2008.
Published in Russian in Fiziologiya Rastenii, 2009, Vol. 56, No. 1, pp. 131–138.
Ubiquitous ribosomes catalyze the peptidyl trans-
ferase reaction of polypeptide synthesis essential for
cellular growth and differentiation in all living cells.
The cytosolic ribosomes of eukaryotes are composed of
a large number of ribosomal proteins (RPs) and four
distinct rRNAs, 18S rRNA of the 40S subunit and the
5S, 5.8S, and 23S-like (25–28S) rRNAs of the 60S sub-
unit . The mouse, human, and
genomes encode all 79 RPs  and 74 RPs from
the 80 predicted RPs were identiﬁed in
ribosomes, including 30 small subunit and 44 large sub-
unit proteins [3, 4].
Association and interaction of RPs within the ribo-
some are closely related to translational control. RPs
are known to be structural elements of the ribosome;
they also to possess a number of extra-ribosomal func-
tions at many stages of various intracellular processes.
Several RP genes are reported to be regulated in plants
both developmentally and environmentally [5–7]. In
soybean, three RP genes were reported to be induced by
low-temperature stress . De novo protein synthesis is
The text was submitted by the authors in English.
These authors contributed equally to the work.
necessary for cold response, and the integrity of the
translation machinery is an important factor of cold
acclimation. It has been reported that several cold-sen-
sitive mutants in yeast are defective in the assembly of
ribosomal subunits [9, 10].
CsdA is a protein
associated with ribosomes at low temperature and has
helix-destabilizing activity .
Ribosomal protein S6 (RPS6) is a major component
of the 43S rRNA preinitiation complex . It seems
is an important housekeeping gene since a
defect in RPS6 causes abnormal development of
hematopoietic cells in
 and partial sup-
resulted in reduced
apical dominance and irregular positioning of leaves
and ﬂowers . Ruvinsky and Meyhas  reported
that RPS6 is involved in cell size determination, protein
synthesis, and glucose homeostasis.
The goal of this study was (1) to clone the genomic
DNA and (2) to carry out the molecular func-
tional analysis of
MATERIALS AND METHODS
Plant materials and treatments.
, cv. Sinpaldal 2) plants were grown in a growth
, an illuminance of 22000 lx, and a 16-h
photoperiod for 3–4 weeks. To induce salt stress, plants
Cloning and Characterization of
Protein S6 in Soybean
, E. Chung
, C. W. Cho
, H. A. Soh
, B. H. Yun
, J. I. Kim
, Y. W. Seo
S. W. Lee
, Y. Ch. Lee
, Y. S. Chung
, and J. H. Lee
BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University,
Busan 604-714, Republic of Korea;
School of Food and Life Science, Biohealth Products Research Center, Inje University, Kimhae 621-749, Republic of Korea
Korea University, Division of Biotechnology, Seoul 136-701, Republic of Korea
Received September 26, 2007
— We isolated and characterized a stress-inducible gene designated as
protein S6 in soybean. The derived amino acid sequence of SLTI98 showed the highest identity of 93% with
ribosomal protein S6 from
(ABD32373). The size of the full-length genomic clone of
is 2701 bp containing 6 exons and 5 introns, of which structure is similar to that of
mal protein S6. Genomic southern-blot analysis conﬁrmed that soybean genome has multiple copies of the
RNA expression was slightly induced by salt, ABA, or wounding stress, but not by dehy-
dration stress. The present study implies that the nuclear
plays an important role in translational control
during abiotic stresses.
Key words: Glycine max - ribosomal protein - stress - northern blot
: GFP—green ﬂuorescent protein; ORF—open read-
ing frame; RP—ribosomal protein; UTR—untranslated region.