Cleavage sites of the “P3 region” in the nonstructural polyprotein precursor of a dicistrovirus

Cleavage sites of the “P3 region” in the nonstructural polyprotein precursor of a dicistrovirus Plautia stali intestine virus (PSIV) is a member of the family Dicistroviridae . Dicistroviruses, like picornaviruses, are thought to encode a 3C-like protease; however, the cleavage sites of dicistroviral nonstructural polyprotein precursors are unknown except for those in genome-linked virus protein (VPg) regions. We expressed part of the PSIV polyprotein in Escherichia coli and detected autoprocessed viral proteins. N-terminal sequence analysis of the autoprocessed proteins showed that Q/GWLSW and Q/NGVFD in the PSIV sequence correspond to 2C/3A and 3C/3D cleavage sites in picornaviruses. Alignments of deduced amino acid sequences of the “P3 region” suggest that these cleavage sites can be predicted. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Cleavage sites of the “P3 region” in the nonstructural polyprotein precursor of a dicistrovirus

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Publisher
Springer Vienna
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-008-0208-5
Publisher site
See Article on Publisher Site

Abstract

Plautia stali intestine virus (PSIV) is a member of the family Dicistroviridae . Dicistroviruses, like picornaviruses, are thought to encode a 3C-like protease; however, the cleavage sites of dicistroviral nonstructural polyprotein precursors are unknown except for those in genome-linked virus protein (VPg) regions. We expressed part of the PSIV polyprotein in Escherichia coli and detected autoprocessed viral proteins. N-terminal sequence analysis of the autoprocessed proteins showed that Q/GWLSW and Q/NGVFD in the PSIV sequence correspond to 2C/3A and 3C/3D cleavage sites in picornaviruses. Alignments of deduced amino acid sequences of the “P3 region” suggest that these cleavage sites can be predicted.

Journal

Archives of VirologySpringer Journals

Published: Oct 1, 2008

References

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