Cl− Channels in Basolateral TAL Membranes. XIX. Cytosolic Cl− Regulates mmClC-Ka and mcClC-Ka Channels

Cl− Channels in Basolateral TAL Membranes. XIX. Cytosolic Cl− Regulates mmClC-Ka and mcClC-Ka... We evaluated the effects of culturing mouse MTAL cells under conditions that suppressed steady-state cytosolic Cl− on chloride channels fused into bilayers from basolateral vesicles of cultured MTAL cells. We used two agents to suppress Cl− entry: 10−6 M PGE2 and 10−4 M bumetanide. Basolateral Cl− channels from control cultured MTAL cells exhibited the signature characteristics of mmClC-Ka channels: increased open-time probability (P o) either by raising cytosolic-face [Cl−] or, at 2 mM cytosolic Cl−, by adding (ATP + PKA), and first-order conductance kinetics. Either 10−6 M PGE2 or 10−4 M bumetanide in culture media reduced steady-state MTAL cytosolic Cl−. Chloride channels from these cells exhibited characteristics unique to CTAL mcClC-Ka channels, namely: no augmentation of P o either by raising cytosolic Cl− or with cytosolic (ATP + PKA), and multi-ion occupancy. Semi-quantitative RT-PCR and real-time quantitative PCR showed that culturing MTAL cells with 10−6 M PGE2 or 10−4 M bumetanide reduced mRNA levels encoding mmClC-Ka but not mRNA levels encoding mcClC-Ka. However, when MTAL cells were cultured under control conditions, and then pre-incubated for 60 minutes with 10−4 M bumetanide, cytosolic Cl− fell acutely but Cl− channels exhibited characteristics of mmClC-Ka channels. Thus PGE2 and bumetanide, both of which lower steady-state MTAL cytosolic Cl− concentrations, inhibit either the transcriptional and/or the translational processes for mmClC-Ka synthesis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Cl− Channels in Basolateral TAL Membranes. XIX. Cytosolic Cl− Regulates mmClC-Ka and mcClC-Ka Channels

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Publisher
Springer-Verlag
Copyright
Copyright © 2003 by Springer-Verlag New York Inc.
Subject
Philosophy
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-003-2046-4
Publisher site
See Article on Publisher Site

Abstract

We evaluated the effects of culturing mouse MTAL cells under conditions that suppressed steady-state cytosolic Cl− on chloride channels fused into bilayers from basolateral vesicles of cultured MTAL cells. We used two agents to suppress Cl− entry: 10−6 M PGE2 and 10−4 M bumetanide. Basolateral Cl− channels from control cultured MTAL cells exhibited the signature characteristics of mmClC-Ka channels: increased open-time probability (P o) either by raising cytosolic-face [Cl−] or, at 2 mM cytosolic Cl−, by adding (ATP + PKA), and first-order conductance kinetics. Either 10−6 M PGE2 or 10−4 M bumetanide in culture media reduced steady-state MTAL cytosolic Cl−. Chloride channels from these cells exhibited characteristics unique to CTAL mcClC-Ka channels, namely: no augmentation of P o either by raising cytosolic Cl− or with cytosolic (ATP + PKA), and multi-ion occupancy. Semi-quantitative RT-PCR and real-time quantitative PCR showed that culturing MTAL cells with 10−6 M PGE2 or 10−4 M bumetanide reduced mRNA levels encoding mmClC-Ka but not mRNA levels encoding mcClC-Ka. However, when MTAL cells were cultured under control conditions, and then pre-incubated for 60 minutes with 10−4 M bumetanide, cytosolic Cl− fell acutely but Cl− channels exhibited characteristics of mmClC-Ka channels. Thus PGE2 and bumetanide, both of which lower steady-state MTAL cytosolic Cl− concentrations, inhibit either the transcriptional and/or the translational processes for mmClC-Ka synthesis.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Oct 9, 2003

References

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