Access the full text.
Sign up today, get DeepDyve free for 14 days.
D Sterling (2002)
10.1152/ajpcell.00115.2002Am J Physiol Cell Physiol, 283
K Lefkimmiatis, M Srikanthan, I Maiellaro, MP Moyer, S Curci, AM Hofer (2009)
Store-operated cyclic AMP signalling mediated by STIM1Nat Cell Biol, 11
JH Shin, EJ Son, HS Lee, SJ Kim, K Kim, JY Choi, MG Lee, JH Yoon (2007)
Molecular and functional expression of anion exchangers in cultured normal human nasal epithelial cellsActa Physiol (Oxf), 191
J Shan (2012)
10.1113/jphysiol.2012.236893J Physiol, 590
M Salvi (2006)
10.1016/j.febslet.2006.06.031FEBS Lett, 580
W Namkung, WE Finkbeiner, AS Verkman (2010)
CFTR-adenylyl cyclase I association responsible for UTP activation of CFTR in well-differentiated primary human bronchial cell culturesMol Biol Cell, 21
MN Chernova (2003)
10.1152/ajpcell.00522.2002Am J Physiol Cell Physiol, 284
JY Choi (2007)
10.1172/JCI31992J Clin Invest, 117
M Soleimani, T Greeley, S Petrovic, Z Wang, H Amlal, P Kopp, CE Burnham (2001)
Pendrin: an apical Cl−/OH−/HCO3 − exchanger in the kidney cortexAm J Physiol Renal Physiol, 280
JH Shin (2007)
10.1111/j.1748-1716.2007.01731.xActa Physiol (Oxf), 191
A Mehta (2008)
Cystic fibrosis as a bowel cancer syndrome and the potential role of CK2Mol Cell Biochem, 316
S Kang, M Xu, EC Cooper, N Hoshi (2014)
Channel-anchored protein kinase CK2 and protein phosphatase 1 reciprocally regulate KCNQ2-containing M-channels via phosphorylation of calmodulinJ Biol Chem, 289
BM Rollins (2008)
10.1165/rcmb.2007-0450OCAm J Respir Cell Mol Biol, 39
H Matsui (1998)
10.1172/JCI2687J Clin Invest, 102
KJ Treharne (2009)
10.1159/000257427Cell Physiol Biochem, 24
P Hegyi, MA Gray, BE Argent (2003)
Substance P inhibits bicarbonate secretion from guinea pig pancreatic ducts by modulating an anion exchangerAm J Physiol Cell Physiol, 285
S Luz (2011)
10.1128/MCB.05517-11Mol Cell Biol, 31
AM Paradiso, RD Coakley, RC Boucher (2003)
Polarized distribution of HCO3 − transport in human normal and cystic fibrosis nasal epitheliaJ Physiol, 548
AK Stewart (2007)
10.1113/jphysiol.2007.136119J Physiol, 584
L Cesaro (2013)
10.1007/s00726-013-1613-yAmino Acids, 45
CM Liedtke, D Cody, TS Cole (2001)
Differential regulation of Cl− transport proteins by PKC in Calu-3 cellsAm J Physiol Lung Cell Mol Physiol, 280
S Luz, P Kongsuphol, AI Mendes, F Romeiras, M Sousa, R Schreiber, P Matos, P Jordan, A Mehta, MD Amaral, K Kunzelmann, CM Farinha (2011)
Contribution of casein kinase 2 and spleen tyrosine kinase to CFTR trafficking and protein kinase A-induced activityMol Cell Biol, 31
M Quadroni, P James, E Carafoli (1994)
Isolation of phosphorylated calmodulin from rat liver and identification of the in vivo phosphorylation sitesJ Biol Chem, 269
J Huang, J Shan, D Kim, J Liao, A Evagelidis, SL Alper, JW Hanrahan (2012)
Basolateral chloride loading by the anion exchanger type 2: role in fluid secretion by the human airway epithelial cell line Calu-3J Physiol, 590
F Pierre, PC Chua, SE O'Brien, A Siddiqui-Jain, P Bourbon, M Haddach, J Michaux, J Nagasawa, MK Schwaebe, E Stefan, A Vialettes, JP Whitten, TK Chen, L Darjania, R Stansfield, K Anderes, J Bliesath, D Drygin, C Ho, M Omori, C Proffitt, N Streiner, K Trent, WG Rice, DM Ryckman (2011)
Discovery and SAR of 5-(3-chlorophenylamino)benzo[c][2,6]naphthyridine-8-carboxylic acid (CX-4945), the first clinical stage inhibitor of protein kinase CK2 for the treatment of cancerJ Med Chem, 54
L Cesaro, O Marin, A Venerando, A Donella-Deana, LA Pinna (2013)
Phosphorylation of cystic fibrosis transmembrane conductance regulator (CFTR) serine-511 by the combined action of tyrosine kinases and CK2: the implication of tyrosine-512 and phenylalanine-508Amino Acids, 45
H Matsui, SH Randell, SW Peretti, CW Davis, RC Boucher (1998)
Coordinated clearance of periciliary liquid and mucus from airway surfacesJ Clin Invest, 102
SK Inglis, L Finlay, SJ Ramminger, K Richard, MR Ward, SM Wilson, RE Olver (2002)
Regulation of intracellular pH in Calu-3 human airway cellsJ Physiol, 538
AK Stewart (2011)
10.1152/ajpcell.00089.2011Am J Physiol Cell Physiol, 301
SB Ko (2004)
10.1038/ncb1115Nat Cell Biol, 6
R Tarran, L Trout, SH Donaldson, RC Boucher (2006)
Soluble mediators, not cilia, determine airway surface liquid volume in normal and cystic fibrosis superficial airway epitheliaJ Gen Physiol, 127
AK Stewart (2002)
10.1085/jgp.20028641J Gen Physiol, 120
FJ Al-Bazzaz, N Hafez, S Tyagi, CA Gailey, M Toofanfard, WA Alrefai, TM Nazir, K Ramaswamy, PK Dudeja (2001)
Detection of Cl--HCO3- and Na+−H+ exchangers in human airways epitheliumJOP, 2
JA Kellum (2000)
10.1186/cc644Crit Care, 4
AM Paradiso (2003)
10.1113/jphysiol.2002.034447J Physiol, 548
PM Quinton (2008)
10.1016/S0140-6736(08)61162-9Lancet, 372
Y Tian (2011)
10.1096/fj.10-166884FASEB J, 25
HW Kim, SH Ha, MN Lee, E Huston, DH Kim, SK Jang, PG Suh, MD Houslay, SH Ryu (2010)
Cyclic AMP controls mTOR through regulation of the dynamic interaction between Rheb and phosphodiesterase 4DMol Cell Biol, 30
D Sterling, NJ Brown, CT Supuran, JR Casey (2002)
The functional and physical relationship between the DRA bicarbonate transporter and carbonic anhydrase IIAm J Physiol Cell Physiol, 283
J Huang (2012)
10.1113/jphysiol.2012.236919J Physiol, 590
MN Chernova, AK Stewart, L Jiang, DJ Friedman, YZ Kunes, SL Alper (2003)
Structure-function relationships of AE2 regulation by Ca(i)(2+)-sensitive stimulators NH(4+) and hypertonicityAm J Physiol Cell Physiol, 284
AL Garland (2013)
10.1073/pnas.1311999110Proc Natl Acad Sci U S A, 110
D Kim (2014)
10.1152/ajpcell.00083.2014Am J Physiol Cell Physiol, 307
G Arrigoni (2004)
10.1021/bi049365cBiochemistry, 43
VS Shah, DK Meyerholz, XX Tang, L Reznikov, M Abou Alaiwa, SE Ernst, PH Karp, CL Wohlford-Lenane, KP Heilmann, MR Leidinger, PD Allen, J Zabner, PB McCray, LS Ostedgaard, DA Stoltz, CO Randak, MJ Welsh (2016)
Airway acidification initiates host defense abnormalities in cystic fibrosis miceScience, 351
R Tarran, B Button, RC Boucher (2006)
Regulation of normal and cystic fibrosis airway surface liquid volume by phasic shear stressAnnu Rev Physiol, 68
JJ Smith, MJ Welsh (1992)
cAMP stimulates bicarbonate secretion across normal, but not cystic fibrosis airway epitheliaJ Clin Invest, 89
A Venerando, M Ruzzene, LA Pinna (2014)
Casein kinase: the triple meaning of a misnomerBiochem J, 460
VS Shah (2016)
10.1126/science.aad5589Science, 351
JL Kreindler (2006)
10.1016/j.bbadis.2006.06.005Biochim Biophys Acta, 1762
BD Humphreys (1994)
10.1152/ajpcell.1994.267.5.C1295Am J Phys, 267
S Petrovic, S Barone, J Xu, L Conforti, L Ma, M Kujala, J Kere, M Soleimani (2004)
SLC26A7: a basolateral Cl−/HCO3- exchanger specific to intercalated cells of the outer medullary collecting ductAm J Physiol Renal Physiol, 286
CM Liedtke (2001)
10.1152/ajplung.2001.280.4.L739Am J Physiol Lung Cell Mol Physiol, 280
JA Kellum (2000)
Determinants of blood pH in health and diseaseCrit Care, 4
R Tarran (2006)
10.1146/annurev.physiol.68.072304.112754Annu Rev Physiol, 68
JY Choi, NS Joo, ME Krouse, JV Wu, RC Robbins, JP Ianowski, JW Hanrahan, JJ Wine (2007)
Synergistic airway gland mucus secretion in response to vasoactive intestinal peptide and carbachol is lost in cystic fibrosisJ Clin Invest, 117
D Kim, J Kim, B Burghardt, L Best, MC Steward (2014)
Role of anion exchangers in Cl− and HCO3− secretion by the human airway epithelial cell line Calu-3Am J Physiol Cell Physiol, 307
J Shan, J Liao, J Huang, R Robert, ML Palmer, SC Fahrenkrug, SM O'Grady, JW Hanrahan (2012)
Bicarbonate-dependent chloride transport drives fluid secretion by the human airway epithelial cell line Calu-3J Physiol, 590
AA Pezzulo (2012)
10.1038/nature11130Nature, 487
S Sarno, M Salvi, R Battistutta, G Zanotti, LA Pinna (2005)
Features and potentials of ATP-site directed CK2 inhibitorsBiochim Biophys Acta, 1754
G Pena-Munzenmayer (2015)
10.1074/jbc.M114.612895J Biol Chem, 290
J Loffing (2000)
10.1152/ajpcell.2000.279.4.C1016Am J Physiol Cell Physiol, 279
A Roos (1981)
10.1152/physrev.1981.61.2.296Physiol Rev, 61
MJ Hoegger, AJ Fischer, JD McMenimen, LS Ostedgaard, AJ Tucker, MA Awadalla, TO Moninger, AS Michalski, EA Hoffman, J Zabner, DA Stoltz, MJ Welsh (2014)
Impaired mucus detachment disrupts mucociliary transport in a piglet model of cystic fibrosisScience, 345
G Pena-Munzenmayer, MA Catalan, Y Kondo, Y Jaramillo, F Liu, GE Shull, JE Melvin (2015)
Ae4 (Slc4a9) anion exchanger drives Cl- uptake-dependent fluid secretion by mouse submandibular gland acinar cellsJ Biol Chem, 290
PK Dudeja, N Hafez, S Tyagi, CA Gailey, M Toofanfard, WA Alrefai, TM Nazir, K Ramaswamy, FJ Al-Bazzaz (1999)
Expression of the Na+/H+ and Cl−/HCO-3 exchanger isoforms in proximal and distal human airwaysAm J Phys, 276
S Kang (2014)
10.1074/jbc.M113.528497J Biol Chem, 289
A Venerando (2014)
10.1042/BJ20140178Biochem J, 460
Y Tian, P Kongsuphol, M Hug, J Ousingsawat, R Witzgall, R Schreiber, K Kunzelmann (2011)
Calmodulin-dependent activation of the epithelial calcium-dependent chloride channel TMEM16AFASEB J, 25
D Borowitz (2015)
10.1002/ppul.23247Pediatr Pulmonol, 50
JP Garnett (2013)
10.1111/bph.12085Br J Pharmacol, 168
M Quadroni (1994)
10.1016/S0021-9258(17)33980-7J Biol Chem, 269
S Petrovic (2003)
10.1152/ajpgi.00454.2002Am J Physiol Gastrointest Liver Physiol, 284
F Pierre (2011)
10.1021/jm101251qJ Med Chem, 54
HV Nguyen (2004)
10.1152/ajpgi.00158.2003Am J Physiol Gastrointest Liver Physiol, 286
M Xie, TC Rich, C Scheitrum, M Conti, W Richter (2011)
Inactivation of multidrug resistance proteins disrupts both cellular extrusion and intracellular degradation of cAMPMol Pharmacol, 80
JL Kreindler, KW Peters, RA Frizzell, RJ Bridges (2006)
Identification and membrane localization of electrogenic sodium bicarbonate cotransporters in Calu-3 cellsBiochim Biophys Acta, 1762
MC Lee, CM Penland, JH Widdicombe, JJ Wine (1998)
Evidence that Calu-3 human airway cells secrete bicarbonateAm J Phys, 274
M Soleimani (2001)
10.1152/ajprenal.2001.280.2.F356Am J Physiol Renal Physiol, 280
PM Quinton (2008)
Cystic fibrosis: impaired bicarbonate secretion and mucoviscidosisLancet, 372
JP Garnett (2011)
10.1074/jbc.M111.266734J Biol Chem, 286
M Schmidt (2013)
10.1124/pr.110.003707Pharmacol Rev, 65
M Schmidt, FJ Dekker, H Maarsingh (2013)
Exchange protein directly activated by cAMP (epac): a multidomain cAMP mediator in the regulation of diverse biological functionsPharmacol Rev, 65
HW Kim (2010)
10.1128/MCB.00217-10Mol Cell Biol, 30
RJ Lee (2010)
10.1172/JCI42992J Clin Invest, 120
F Meggio (2004)
10.1021/bi048999gAn in vitro and in vivo study Biochemistry, 43
S Petrovic (2004)
10.1152/ajprenal.00219.2003Am J Physiol Renal Physiol, 286
F Meggio, MA Pagano, S Moro, G Zagotto, M Ruzzene, S Sarno, G Cozza, J Bain, M Elliott, AD Deana, AM Brunati, LA Pinna (2004)
Inhibition of protein kinase CK2 by condensed polyphenolic derivativesAn in vitro and in vivo study Biochemistry, 43
P Hegyi (2003)
10.1152/ajpcell.00574.2002Am J Physiol Cell Physiol, 285
HV Nguyen, A Stuart-Tilley, SL Alper, JE Melvin (2004)
Cl(−)/HCO(3)(−) exchange is acetazolamide sensitive and activated by a muscarinic receptor-induced [Ca(2+)](i) increase in salivary acinar cellsAm J Physiol Gastrointest Liver Physiol, 286
S Sarno (2005)
10.1016/j.bbapap.2005.07.043Biochim Biophys Acta, 1754
ST Ballard, L Trout, Z Bebok, EJ Sorscher, A Crews (1999)
CFTR involvement in chloride, bicarbonate, and liquid secretion by airway submucosal glandsAm J Phys, 277
KR Bhaskar, DH Gong, R Bansil, S Pajevic, JA Hamilton, BS Turner, JT LaMont (1991)
Profound increase in viscosity and aggregation of pig gastric mucin at low pHAm J Phys, 261
RD Coakley (2003)
10.1073/pnas.2634339100Proc Natl Acad Sci U S A, 100
DC Devor (1999)
10.1085/jgp.113.5.743J Gen Physiol, 113
BD Humphreys, L Jiang, MN Chernova, SL Alper (1994)
Functional characterization and regulation by pH of murine AE2 anion exchanger expressed in Xenopus oocytesAm J Phys, 267
G Arrigoni, O Marin, MA Pagano, L Settimo, B Paolin, F Meggio, LA Pinna (2004)
Phosphorylation of calmodulin fragments by protein kinase CK2. Mechanistic aspects and structural consequencesBiochemistry, 43
S Petrovic, X Ju, S Barone, U Seidler, SL Alper, H Lohi, J Kere, M Soleimani (2003)
Identification of a basolateral Cl−/HCO3- exchanger specific to gastric parietal cellsAm J Physiol Gastrointest Liver Physiol, 284
J Loffing, BD Moyer, D Reynolds, BE Shmukler, SL Alper, BA Stanton (2000)
Functional and molecular characterization of an anion exchanger in airway serous epithelial cellsAm J Physiol Cell Physiol, 279
DC Devor, AK Singh, LC Lambert, A DeLuca, RA Frizzell, RJ Bridges (1999)
Bicarbonate and chloride secretion in Calu-3 human airway epithelial cellsJ Gen Physiol, 113
RD Coakley, BR Grubb, AM Paradiso, JT Gatzy, LG Johnson, SM Kreda, WK O'Neal, RC Boucher (2003)
Abnormal surface liquid pH regulation by cultured cystic fibrosis bronchial epitheliumProc Natl Acad Sci U S A, 100
AK Stewart, CE Kurschat, RD Vaughan-Jones, BE Shmukler, SL Alper (2007)
Acute regulation of mouse AE2 anion exchanger requires isoform-specific amino acid residues from most of the transmembrane domainJ Physiol, 584
A Roos, WF Boron (1981)
Intracellular pHPhysiol Rev, 61
SL Alper, RB Darman, MN Chernova, NK Dahl (2002)
The AE gene family of Cl/HCO3- exchangersJ Nephrol, 15
JP Garnett, E Hickman, O Tunkamnerdthai, AW Cuthbert, MA Gray (2013)
Protein phosphatase 1 coordinates CFTR-dependent airway epithelial HCO3 − secretion by reciprocal regulation of apical and basolateral membrane Cl−-HCO3 − exchangersBr J Pharmacol, 168
A Mehta (2008)
10.1007/s11010-008-9815-4Mol Cell Biochem, 316
JJ Smith (1992)
10.1172/JCI115696J Clin Invest, 89
KJ Treharne, Z Xu, JH Chen, OG Best, DM Cassidy, DC Gruenert, P Hegyi, MA Gray, DN Sheppard, K Kunzelmann, A Mehta (2009)
Inhibition of protein kinase CK2 closes the CFTR Cl channel, but has no effect on the cystic fibrosis mutant deltaF508-CFTRCell Physiol Biochem, 24
SK Inglis (2002)
10.1113/jphysiol.2001.012806J Physiol, 538
BS Lee, RB Gunn, RR Kopito (1991)
Functional differences among nonerythroid anion exchangers expressed in a transfected human cell lineJ Biol Chem, 266
BS Lee (1991)
10.1016/S0021-9258(18)98979-9J Biol Chem, 266
RJ Lee, JK Foskett (2010)
cAMP-activated Ca2+ signaling is required for CFTR-mediated serous cell fluid secretion in porcine and human airwaysJ Clin Invest, 120
JP Garnett, E Hickman, R Burrows, P Hegyi, L Tiszlavicz, AW Cuthbert, P Fong, MA Gray (2011)
Novel role for pendrin in orchestrating bicarbonate secretion in cystic fibrosis transmembrane conductance regulator (CFTR)-expressing airway serous cellsJ Biol Chem, 286
AL Garland, WG Walton, RD Coakley, CD Tan, RC Gilmore, CA Hobbs, A Tripathy, LA Clunes, S Bencharit, MJ Stutts, L Betts, MR Redinbo, R Tarran (2013)
Molecular basis for pH-dependent mucosal dehydration in cystic fibrosis airwaysProc Natl Acad Sci U S A, 110
MJ Hoegger (2014)
10.1126/science.1255825Science, 345
M Xie (2011)
10.1124/mol.111.071134Mol Pharmacol, 80
AA Pezzulo, XX Tang, MJ Hoegger, MH Alaiwa, S Ramachandran, PH Karp, CL Wohlford-Lenane, HP Haagsman, M Eijk, B Banfi, AR Horswill, DA Stoltz, PB McCray, MJ Welsh, J Zabner (2012)
Reduced airway surface pH impairs bacterial killing in the porcine cystic fibrosis lungNature, 487
D Borowitz (2015)
CFTR, bicarbonate, and the pathophysiology of cystic fibrosisPediatr Pulmonol, 50
S Moon, M Singh, ME Krouse, JJ Wine (1997)
Calcium-stimulated Cl− secretion in Calu-3 human airway cells requires CFTRAm J Phys, 273
BM Rollins, M Burn, RD Coakley, LA Chambers, AJ Hirsh, MT Clunes, MI Lethem, SH Donaldson, R Tarran (2008)
A2B adenosine receptors regulate the mucus clearance component of the lung’s innate defense systemAm J Respir Cell Mol Biol, 39
SB Ko, W Zeng, MR Dorwart, X Luo, KH Kim, L Millen, H Goto, S Naruse, A Soyombo, PJ Thomas, S Muallem (2004)
Gating of CFTR by the STAS domain of SLC26 transportersNat Cell Biol, 6
AK Stewart, BE Shmukler, DH Vandorpe, F Reimold, JF Heneghan, M Nakakuki, A Akhavein, S Ko, H Ishiguro, SL Alper (2011)
SLC26 anion exchangers of guinea pig pancreatic duct: molecular cloning and functional characterizationAm J Physiol Cell Physiol, 301
W Namkung (2010)
10.1091/mbc.E09-12-1004Mol Biol Cell, 21
AK Stewart, MN Chernova, BE Shmukler, S Wilhelm, SL Alper (2002)
Regulation of AE2-mediated Cl- transport by intracellular or by extracellular pH requires highly conserved amino acid residues of the AE2 NH2-terminal cytoplasmic domainJ Gen Physiol, 120
K Lefkimmiatis (2009)
10.1038/ncb1850Nat Cell Biol, 11
R Tarran (2006)
10.1085/jgp.200509468J Gen Physiol, 127
M Salvi, S Sarno, O Marin, F Meggio, E Itarte, LA Pinna (2006)
Discrimination between the activity of protein kinase CK2 holoenzyme and its catalytic subunitsFEBS Lett, 580
Transepithelial bicarbonate secretion by human airway submucosal glands and surface epithelial cells is crucial to maintain the pH-sensitive innate defence mechanisms of the lung. cAMP agonists stimulate HCO3 − secretion via coordinated increases in basolateral HCO3 − influx and accumulation, as well as CFTR-dependent HCO3 − efflux at the luminal membrane of airway epithelial cells. Here, we investigated the regulation of a basolateral located, DIDS-sensitive, Cl−/HCO3 − exchanger, anion exchanger 2 (AE2; SLC4A2) which is postulated to act as an acid loader, and therefore potential regulator of HCO3 − secretion, in human airway epithelial cells. Using intracellular pH measurements performed on Calu-3 cells, we demonstrate that the activity of the basolateral Cl−/HCO3 − exchanger was significantly downregulated by cAMP agonists, via a PKA-independent mechanism and also required Ca2+ and calmodulin under resting conditions. AE2 contains potential phosphorylation sites by a calmodulin substrate, protein kinase CK2, and we demonstrated that AE2 activity was reduced in the presence of CK2 inhibition. Moreover, CK2 inhibition abolished the activity of AE2 in primary human nasal epithelia. Studies performed on mouse AE2 transfected into HEK-293T cells confirmed almost identical Ca2+/calmodulin and CK2 regulation to that observed in Calu-3 and primary human nasal cells. Furthermore, mouse AE2 activity was reduced by genetic knockout of CK2, an effect which was rescued by exogenous CK2 expression. Together, these findings are the first to demonstrate that CK2 is a key regulator of Cl−-dependent HCO3 − export at the serosal membrane of human airway epithelial cells.
Pflügers Archiv European Journal of Physiologyl of Physiology – Springer Journals
Published: Apr 28, 2017
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.