Chromosome mapping of the Emr1 gene

Chromosome mapping of the Emr1 gene 946 Mammalian Genome 8, Brief Data Reports Chromosome mapping of the Emrl gene Andrew J. McKnight, a Alison J. Macfarlane, 1 o..,,, 9 [] 9 [] 9 [] DlTMit7 Michael F. Seldin, 2 Siamon Gordon 1 5.3 +_ 2.1 clVl Emrl, DlTMit20 [] [] [] 9 9 [] Emrl, DlTMit20 2.6  1.$ cM ~Sir William Dunn School of Pathology, University of Oxford, South [] [] [] 9 [] 9 -- ~ Hprt-psl Parks Road, Oxford, OX1 3RE, England, UK 2Rowe Program in Genetics, Departments of Biological Chemistry and # of mice 55 54 3 3 1 2 Medicine, University of California at Davis, Davis, California 95616, USA Fig. 1. Segregation of Emrl on mouse Chr 17 in [(C3I-I/HeJ-g/d x Mus Received: 27 June 1997 / Accepted: 22 July 1997 spretus)F 1 x C3H/HeJ-gld] interspecific backcross mice. C3HIHeLgld, Mus spretus (Spain) mice and [(C3HIHeJ-gld x Mus spretus)F 1 x C31-II Species: Mouse HeJ-gld] interspecific backcross mice were bred and maintained as previ- Locus name: EGF-module-containing mucin-like hormone recep- ously described [7]. Mus spretus was chosen as the second parent in this cross because of the relative ease of detection of informative RFLVs in tor 1 comparison with crosses using conventional inbred laboratory strains. Locus symbol: Emrl DNA isolated from mouse organs by standard techniques was digested Map position: The distal region of mouse Chromosome (Chr) 17: with restriction endonucleases and 10 txg samples were electrophoresed in (centromere) D17Mit7-5.3 + 2.1 cM-Emrl-2.6 +_ 1.5 cM-Hprt- 0.9% agarose gels. DNA was transferred to Nytran membranes (Schleicher psl. & Schull, Inc., Keene, NH), hybridized at 65~ to probes labeled by the Method of mapping: DNA from two parental strains [C3H/HeJ- random primed method with [32p]-dCTP and washed under stringent con- gld and (C3H/HeJ-g/d x Mus spretus)F1] were digested with vari- ditions, all as previously described [8]. Gene linkage was determined by ous restriction endonucleases and hybridized with an F4/80 cDNA segregation analysis [9]. Gene order was determined by analyzing all hap- probe to determine restriction fragment length variants (RFLVs) to lotypes and minimizing corssover frequency between all genes that were determined to be within a linkage group. Filled boxes represent the homo- allow haplotype analyses. zygous C3H pattern and open boxes the F 1 pattern. The mapping of the Database deposit information: MGD-JNUM-40392 reference loci [D17Mit7 and hypoxanthine phosphoribosyl transferase Molecular reagents used for mapping: cDNA probe derived from pseudogene 1 (ttprt-psl, formerly D17RPIO)] in this interspecific cross clone pF4/80(12.2) has been described previously [10]. Method of allele detection and nature of aUelic variant: BglII RFLVs were detected and analyzed: C3H/HeJ-gld 11.0 kb, 6.7 kb, and 3.4 kb; Mus spretus 16.0 kb and 8.2 kb. In each of the back- istics, Physiology and Function, R van Furth, ed. (Boston, Dordrecht, cross mice either the C3H/HeJ-gld parental bands or all five bands Lancaster: Martinus Nijboff Publishers), pp 9-17 (two Mus spretus bands and three half-intensity C3H/HeJ-gld 3. McKnight A J, Macfarlane A J, Dri P, Turley L, Willis AC, Gordon S bands) were observed, indicating that a single locus was detected. (1996) J Biol Chem 271,,486-489 Previously identified homolog: EMR1 (human); Chr 19p13.3 4. McKnight AJ, Gordon S (1996) Immunol Today 17, 283-287 Discussion: F4/80 is a 160-kDa glycoprotein expressed on certain populations of mouse tissue macrophages [1,2]. The isolation of 5. Hamann J, Eichler W, Hamann D, Kerstens HMJ, Poddighe P J, Hoovers JMN, Hartmann E, Strauss M, van Lier RAW (1995) J Im- cDNA clones encoding F4/80 demonstrates that the molecule com- munol 155, 1942-1950 prises three subregions: a seven transmemhrane-spanning (TM7) 6. Baud V, Chissoe SL, Viegas-P6quignot E, Diriong S, N'Guyen VC, region, a membrane-proximal extracellular region abundant in ser- Roe BA, Lipinski M (1995) Genomics 26, 334-344 ine and threonine residues, and an NHz-terminal region consisting of seven epidermal growth factor (EGF) domains [3]. This novel 7. Seldin MF, Morse HC, Reeves JP, Scribner JP, LeBoeuf RC, Steinberg AD (1988) J Exp Med 167, 688-693 structure defines a subset of leukocyte-restricted TM7 molecules designated the EGF-TM7 family, which differ in their number of 8. Watson ML, Seldin MF (1994) Methods Molec Genetics 5, 369-387 NHz-terminal EGF domains, the other characterized members of 9. Green EL (1981) In Genetics and Probability in Animal Breeding which are human CD97 and human EGF-module-containing mu- Experiments, E Green, ed. (New York: Macmillan), pp 77-113 cin-like hormone receptor 1 (EMR1) [4]. The human CD97 gene 10. Cazzaniga G, Terao M, Schiavo PL, Galbiati F, Segalla F, Soldin MF, has been mapped to chromosomal region 19p13.12-13.2 [5], and Garattini E (1994) Genomics 23, 390-402 the EMR1 gene, which is the predicted human homolog of mouse F4/80, maps to region 19t>13.3 [6]. This has led to the prediction for clustered EGF-TM7 genes located on the short arm of human Chr 19. To extend our characterization of the mouse F4/80 gene, The Cystatin S gene maps to rat we undertook gene mapping studies described in this report. In Chromosome 3, to which D1mgh18 is accordance with the human nomenclature, the gene encoding the re-assigned from Chromosome 1 F4/80 molecule will hereafter be referred to as Emrl. The position of the EMR1 gene on the short arm of human Chr 19 (19p13.3) extends the homology relationship between this region of the hu- M. Beatriz Duran Alonso, 1 Paul Shiels, 1 man genome and distal mouse Chr 17. Andrew S. McCallion, 1 Neil K. Bennett, 3 Anthony P. Payne, 2 Josiane Szpirer, 4 Claude Szpirer, 4 Acknowledgments: This work was supported by The Medical Research Martin J. Brodie, s R. Wayne Davies, 1 Council, UK, and in part by National Institutes of Health grant HG00734. Roger G. Sutcliffe 1 References 1Division of Molecular Genetics, Institute of Biomedical and Life 1. Austyn JM, Gordon S (1981) Eur J Immunol 11, 805-815 Sciences, The University of Glasgow, Glasgow G12 8QQ, UK 2. Hume DA, Gordon S (1985) In Mononuclear Phagocytes: Character- Correspondence to: A.J. McKnight Correspondence to: R.G. Sutcliffe and R.W. Davis Mammalian Genome Springer Journals

Chromosome mapping of the Emr1 gene

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