Chromosomal assignments of 19 porcine cDNA sequences by FISH

Chromosomal assignments of 19 porcine cDNA sequences by FISH Mammalian Genome 9, 394-396 (1998). Mammalian Genome Incorporating Mouse Oenome © Springer-Verlag New York Inc. 1998 2 Merete Fredholm 2 Preben D. Thomsen,' Anne Katrine WinterO, 'Division of Anatomy, Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, Bulowsvej 13, 1870 Frederiksberg C, Denmark Department of Animal Science and Animal Health, The Royal Veterinary and Agricultural University, Copenhagen, Denmark Received: 13 September 1997 / Accepted: 17 December 1997 A major step towards a comparative map of the porcine genome with one layer of biotinylated anti-avidin antibody (Vector Labo- ratories). The high intensity of fluorescence produced by 20-40 p g was taken by ZOO-FISH chromosome painting that allowed de- termination of synteny conservation between human and pig propidium iodide in alkaline mounting medium, which allows R- (Rettenberger et al. 1995; Fronicke et al. 1996; Goureau et al. banding of BrdU-incorporated chromosomes and simultaneous de- 1996). However, internal rearrangements of gene order within re- tection of FITC signal (Lemieux et al. 1992), did, however, not gions of synteny conservation are not uncovered in these experi- permit the simultaneous detection of the usually weak FITC sig- ments. There is, therefore, still a need to increase the resolution of nals produced Mammalian Genome Springer Journals

Chromosomal assignments of 19 porcine cDNA sequences by FISH

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Copyright © 1998 by Springer-Verlag
Life Sciences; Cell Biology; Anatomy; Zoology
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