9, 243-245 (1998). Mammalian Genome Mammalian Genome Inrorporaling 1lonvr l:rnnmr © Springer-Verlag New York Inc. 1998 Chromosomal assignment of nineteen rat microsatellite markers to various chromosomes by linkage analysis Vladimir E. Sverdlov,' Oksana I. Dukhanina,' Carl Choi,' Marie-Therese Bihoreau, 2 Howard Dene,' John P. Rapp' 'Department of Physiology and Molecular Medicine, Medical College of Ohio, 3000 Arlington Avenue, Toledo, Ohio 43699, USA 2Wellcome Trust Centre for Human Genetics, University of Oxford, Windmill Road, Oxford OX3 7BN, UK Received: 23 July 1997 / Accepted: 29 October 1997 Nineteen polymorphic microsatellite markers obtained by various lected individual fragments, each containing a CTG microsatellite, approaches were mapped onto different rat chromosomes by link- were isolated from the gel, reamplified, and subjected to cycle age analysis. Eleven autosomes are represented by at least one of sequencing with the Amersham Life Science thermo sequenase these markers. Two markers were linked to the X Chromosome cycle sequencing kit (Cleveland, Ohio) and the primer specific to (Chr). Four markers (DIMco9, D7Mco3, D10Mco41, and the adapter. Following sequencing of the initial CTG-containing DIOMco43) were linked to blood pressure quantitative trait loci fragments, primers to perform primer walking (Siebert et al. 1995) (QTL), while others improve the existing
Mammalian Genome – Springer Journals
Published: Mar 21, 2009
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