Cholinergic regulation of body-wall muscle contraction of the ascidian Styela rustica (Linnaeus, 1767)

Cholinergic regulation of body-wall muscle contraction of the ascidian Styela rustica (Linnaeus,... The regulation of body-wall muscle contraction in the ascidian Styela rustica was studied. Acetylcholine (ACh, 1–10 μM) induced a significant contraction of isolated muscle strips. The ACh-induced contractile response was potentiated and prolonged in the presence of proserine (15 μM), which confirms acetylcholinesterase activity in the S. rustica body-wall muscle. Atropine (1–100 μM, M-cholinoreceptor blocker) did not prevent the ACh-induced contractile response, while d-tubocurarine (1–100 μM, N-cholinoreceptor blocker) progressively reduced muscle contraction induced by 10 μM ACh. Thus, neuromuscular transmission in the S. rustica body-wall muscle is mediated by nicotinic-like ACh-receptors. Procaine reduced ACh-induced (10 μM) muscle contraction. As well, our experiments showed spontaneous rhythmic contractile activity in isolated muscle strips of S. rustica. Atropine, d-tubocurarine, procaine, and proserine did not alter rhythmic activity. Myogenic automaticity is suggested as a possible cause of the rhythmic contraction of the ascidian body-wall muscle. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Marine Biology Springer Journals

Cholinergic regulation of body-wall muscle contraction of the ascidian Styela rustica (Linnaeus, 1767)

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2012 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Freshwater & Marine Ecology
ISSN
1063-0740
eISSN
1608-3377
D.O.I.
10.1134/S106307401203008X
Publisher site
See Article on Publisher Site

Abstract

The regulation of body-wall muscle contraction in the ascidian Styela rustica was studied. Acetylcholine (ACh, 1–10 μM) induced a significant contraction of isolated muscle strips. The ACh-induced contractile response was potentiated and prolonged in the presence of proserine (15 μM), which confirms acetylcholinesterase activity in the S. rustica body-wall muscle. Atropine (1–100 μM, M-cholinoreceptor blocker) did not prevent the ACh-induced contractile response, while d-tubocurarine (1–100 μM, N-cholinoreceptor blocker) progressively reduced muscle contraction induced by 10 μM ACh. Thus, neuromuscular transmission in the S. rustica body-wall muscle is mediated by nicotinic-like ACh-receptors. Procaine reduced ACh-induced (10 μM) muscle contraction. As well, our experiments showed spontaneous rhythmic contractile activity in isolated muscle strips of S. rustica. Atropine, d-tubocurarine, procaine, and proserine did not alter rhythmic activity. Myogenic automaticity is suggested as a possible cause of the rhythmic contraction of the ascidian body-wall muscle.

Journal

Russian Journal of Marine BiologySpringer Journals

Published: Jul 4, 2012

References

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