Chitosan-based hybrid immobilization in bienzymatic reactions and its application to the production of laminaribiose

Chitosan-based hybrid immobilization in bienzymatic reactions and its application to the... A hybrid-immobilization method was developed to improve the long-term stability of laminaribiose phosphorylase immobilized on epoxy supports Sepabeads EC-EP/S. Entrapment in chitosan retained all of the enzyme activity depending on the amount of entrapped solid materials and increased half-life by a factor of 10–94.4 h. No enzyme activity loss was determined during 12 times reuse. The immobilization method is also applicable to sucrose phosphorylase immobilized on Sepabeads EC-EP/S. Up to 31.9 g/L laminaribiose were produced in bienzymatic batch experiments with reaction-integrated product separation by adsorption on zeolites. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Bioprocess and Biosystems Engineering Springer Journals

Chitosan-based hybrid immobilization in bienzymatic reactions and its application to the production of laminaribiose

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2017 by Springer-Verlag GmbH Germany
Subject
Chemistry; Biotechnology; Industrial and Production Engineering; Environmental Engineering/Biotechnology; Industrial Chemistry/Chemical Engineering; Food Science
ISSN
1615-7591
eISSN
1615-7605
D.O.I.
10.1007/s00449-017-1797-8
Publisher site
See Article on Publisher Site

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